SF3B1 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2731899

Product Details

Target: SF3B1 (Splicing Factor 3b, Subunit 1, 155kDa (SF3B1))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 2

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SF3B1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human SF3B1 / SAP155 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: SF3B1 (Splicing Factor 3b, Subunit 1, 155kDa (SF3B1))

Alternative Name: Sf3b1,sap155 (SF3B1 Products)

Synonyms: Cus1 Protein, SAP145 Protein, SF3B145 Protein, SF3b1 Protein, SF3b150 Protein, Hsh155 Protein, MDS Protein, PRP10 Protein, PRPF10 Protein, SAP155 Protein, SF3b155 Protein, 155kDa Protein, 2810001M05Rik Protein, AA409119 Protein, Prp10 Protein, TA-8 Protein, Targ4 Protein, hsh155 Protein, prp10 Protein, prpf10 Protein, sap155 Protein, SF3B1 Protein, Sap155 Protein, wu:fb99f09 Protein, splicing factor 3b subunit 2 Protein, splicing factor 3b subunit 1 Protein, splicing factor 3b, subunit 1 Protein, splicing factor 3b subunit 1 S homeolog Protein, SF3B2 Protein, SF3B1 Protein, Sf3b1 Protein, sf3b1.S Protein, sf3b1 Protein

Background: This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 15.8 kDa

NCBI Accession: NP_001005526

Pathways: Chromatin Binding