Pre-mRNA Branch Site Protein p14 (SF3B14) protein (His tag)
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- Target See all Pre-mRNA Branch Site Protein p14 (SF3B14) Proteins
- Pre-mRNA Branch Site Protein p14 (SF3B14)
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human SF3B14 / SAP14 (full length, N-term HIS tag) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product SF3B14 Protein
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Pre-mRNA Branch Site Protein p14 (SF3B14) (AA 1-125) protein (His tag)
SF3B14 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Pre-mRNA Branch Site Protein p14 (SF3B14) (AA 1-125) protein (GST tag)SF3B14 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Pre-mRNA Branch Site Protein p14 (SF3B14) (AA 1-125) protein (Strep Tag)Crystallography grade SF3B14 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 100 mM Arg. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- Pre-mRNA Branch Site Protein p14 (SF3B14)
- Alternative Name
- Sf3b14,sap14 (SF3B14 Products)
- Synonyms
- 6030419K15Rik Protein, AV001342 Protein, Sf3b14 Protein, zgc:86708 Protein, HSPC175 Protein, Ht006 Protein, P14 Protein, SAP14 Protein, SF3B14a Protein, splicing factor 3B, subunit 6 Protein, splicing factor 3b, subunit 6 Protein, splicing factor 3b subunit 6 S homeolog Protein, splicing factor 3b subunit 6 Protein, Sf3b6 Protein, sf3b6 Protein, sf3b6.S Protein, SF3B6 Protein
- Background
- This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.
- Molecular Weight
- 14.6 kDa
- NCBI Accession
- NP_057131
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