SFRP1 Protein
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- Target See all SFRP1 Proteins
- SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HeLa Cells
- Application
- Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human SFRP1 protein expressed in HeLa cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Determined by its ability to inhibit BMP-2 proliferation effect of ATDC-5 cells. The expected ED50 is 0.3-0.5ug/mL.
- Top Product
- Discover our top product SFRP1 Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))
- Alternative Name
- Sfrp1 (SFRP1 Products)
- Synonyms
- FRP Protein, FRP-1 Protein, FRP1 Protein, FrzA Protein, SARP2 Protein, 2210415K03Rik Protein, AW011917 Protein, AW107218 Protein, AW742929 Protein, sFRP-1 Protein, CSFRP1 Protein, SFRP1 Protein, XfrzA Protein, frp Protein, frp-1 Protein, frp1 Protein, frzA Protein, sarp2 Protein, sfrp1 Protein, LOC100231868 Protein, Srfp1a Protein, wu:fd19b09 Protein, secreted frizzled related protein 1 Protein, secreted frizzled-related protein 1 Protein, Secreted frizzled-related protein 1 Protein, secreted frizzled-related protein 1a Protein, secreted frizzled-related protein 1b Protein, SFRP1 Protein, Sfrp1 Protein, sfrp1 Protein, sfrp1a Protein, sfrp1b Protein
- Background
- This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina.
- Molecular Weight
- 31.1 kDa
- NCBI Accession
- NP_003003
- Pathways
- WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation
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