TK1 Protein (DYKDDDDK Tag)
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- Target See all TK1 Proteins
- TK1 (Thymidine Kinase 1, Soluble (TK1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TK1 protein is labelled with DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Thymidine kinase 1 (TK1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product TK1 Protein
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Thymidine Kinase 1, Soluble (TK1) (AA 2-242) protein (His tag)
TK1 Origin: Dog Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Thymidine Kinase 1, Soluble (TK1) (AA 2-234) protein (His tag)TK1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Thymidine Kinase 1, Soluble (TK1) (AA 1-234) protein (GST tag)TK1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Thymidine Kinase 1, Soluble (TK1) (AA 157-234) protein (GST tag)TK1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Thymidine Kinase 1, Soluble (TK1) (AA 2-234) protein (His tag)Crystallography grade TK1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Thymidine Kinase 1, Soluble (TK1) (AA 2-233) protein (His tag)Crystallography grade TK1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Thymidine Kinase 1, Soluble (TK1) (AA 1-376), (full length) protein (His tag)TK1 Origin: Herpes Simplex Virus (HSV) Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- TK1 (Thymidine Kinase 1, Soluble (TK1))
- Alternative Name
- Thymidine Kinase 1 (Tk1) (TK1 Products)
- Synonyms
- fi19b06 Protein, zgc:55362 Protein, wu:fi19b06 Protein, TK1 Protein, tk2 Protein, D530002A18Rik Protein, Tk-1 Protein, Tk1a Protein, Tk1b Protein, TK2 Protein, Tk Protein, TK Protein, thymidine kinase 1, soluble Protein, thymidine kinase 1 Protein, thymidine kinase, cytosolic Protein, Thymidine kinase, cytosolic Protein, temporal expression: early Protein, thymidine kinase Protein, Tk thymidine kinase Protein, tk1 Protein, TK1 Protein, EDI_049980 Protein, kith Protein, J2R Protein, HVT031 Protein, Tk1 Protein, tk Protein
- Background
- The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers.
- Molecular Weight
- 27 kDa
- NCBI Accession
- NP_003249
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