TNFSF15 Protein

Catalog No. ABIN2733959

Product Details

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Protein Interaction (PI), Antibody Production (AbP), Standard (STD)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human TNFSF15 protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: < 0.1 EU per μg protein as determined by LAL test

Biological Activity Comment: ED50 is less than 5 μg/ml as determined by the dose-dependent inhibition of the proliferation of HUVEC cells.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM Phosphate buffer, 250 mM NaCl, pH 7.5. Stable for at least 6 months from date of receipt under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Alternative Name: Tnfsf15 (TNFSF15 Products)

Synonyms: TTPAL Protein, TL1 Protein, TL1A Protein, VEGI Protein, VEGI192A Protein, Tl1 Protein, Tl1a Protein, Vegi Protein, tumor necrosis factor superfamily member 15 Protein, TNF superfamily member 15 Protein, tumor necrosis factor (ligand) superfamily, member 15 Protein, TNFSF15 Protein, Tnfsf15 Protein

Background: The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 21.9 kDa

NCBI Accession: NP_005109

Pathways: Positive Regulation of Endopeptidase Activity, Autophagy