TPSB2 Protein (His tag)
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- Target See all TPSB2 Proteins
- TPSB2 (Tryptase beta 2 (TPSB2))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TPSB2 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human TPSB2 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Top Product
- Discover our top product TPSB2 Protein
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Tryptase beta 2 (TPSB2) (AA 32-276) protein (His tag)
TPSB2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Tryptase beta 2 (TPSB2) (AA 1-275) protein (GST tag)TPSB2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Tryptase beta 2 (TPSB2) protein (His tag)TPSB2 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Tryptase beta 2 (TPSB2) (AA 31-275) protein (His tag)Crystallography grade TPSB2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Tryptase beta 2 (TPSB2) (AA 32-276) protein (His tag)Crystallography grade TPSB2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Buffer
- Supplied as a 0.2 μM filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- TPSB2 (Tryptase beta 2 (TPSB2))
- Alternative Name
- Tryptase beta 2 (Gene/pseudogene) (Tpsb2) (TPSB2 Products)
- Synonyms
- TPS2 Protein, tryptaseB Protein, tryptaseC Protein, Mcpt6 Protein, tryptase beta 2 (gene/pseudogene) Protein, tryptase beta 2 Protein, TPSB2 Protein, Tpsb2 Protein
- Background
- Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, beta II and beta III. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha-tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.
- Molecular Weight
- 29.64kD
- NCBI Accession
- NP_077078
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