TWEAK Protein

Catalog No. ABIN2734639

Product Details

Target: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Protein Interaction (PI), Antibody Production (AbP), Standard (STD)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human TWEAK / TNFSF12 protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: Assay #1: ED50 as determined by the dose-dependent stimulation of IL-8 production by Human PBMC is less than 10 ng/ml. Assay #2: TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-gamma. ED50 for this effect is between 30-45 ng/ml.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handling Advice: Resuspend the protein in the desired concentration in proper buffer

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Alternative Name: Tweak,tnfsf12 (TNFSF12 Products)

Synonyms: APO3L Protein, DR3LG Protein, TWEAK Protein, Apo3l Protein, Dr3l Protein, Dr3lg Protein, Tweak Protein, TNF superfamily member 12 Protein, tumor necrosis factor (ligand) superfamily, member 12 Protein, TNFSF12 Protein, Tnfsf12 Protein

Background: This gene encodes a member of the tumor necrosis factor superfamily. It encodes a hybrid protein composed of the cytoplasmic and transmembrane domains of family member 12 fused to the C-terminal domain of family member 13. The hybrid protein is membrane anchored and presents the receptor-binding domain of family member 13 at the cell surface. It stimulates cycling in T- and B-lymphoma cell lines.

Molecular Weight: 17 kDa

NCBI Accession: NP_742086

Pathways: Apoptosis