UBE2E3 Protein (His tag)

Catalog No. ABIN2734826

Product Details

Target: UBE2E3 (Ubiquitin-Conjugating Enzyme E2E 3 (UBE2E3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UBE2E3 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human UBE2E3 (full length, N-term HIS tag, transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: UBE2E3 (Ubiquitin-Conjugating Enzyme E2E 3 (UBE2E3))

Alternative Name: Ube2e3 (UBE2E3 Products)

Synonyms: UBCH9 Protein, UbcM2 Protein, UbcD2 Protein, wu:fb26h04 Protein, zgc:55923 Protein, zgc:76906 Protein, ubc15 Protein, ubch9 Protein, ubcm2 Protein, xubc15 Protein, Ubce4 Protein, ubcM2 Protein, ube2e3 Protein, ubiquitin conjugating enzyme E2 E3 Protein, ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast) Protein, ubiquitin-conjugating enzyme E2E 3 Protein, Ubiquitin-conjugating enzyme E2 E3 Protein, ubiquitin-conjugating enzyme E2 E3 Protein, ubiquitin conjugating enzyme E2 E3 L homeolog Protein, ubiquitin-conjugating enzyme UBE2E3 Protein, UBE2E3 Protein, ube2e3 Protein, Ube2e3 Protein, ub2e3 Protein, LOC100280942 Protein, LOC100282225 Protein, PTRG_01263 Protein, ube2e3.L Protein, LOC780737 Protein

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100 % sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Molecular Weight: 22.8 kDa

NCBI Accession: NP_006348