RENT1/UPF1 Protein (Myc-DYKDDDDK Tag)
Quick Overview for RENT1/UPF1 Protein (Myc-DYKDDDDK Tag) (ABIN2735034)
Target
See all RENT1/UPF1 (UPF1) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This RENT1/UPF1 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human UPF1 / RENT1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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UPF1 Regulator of Nonsense Transcripts Homolog (UPF1) (AA 1-1124) protein (His tag)
custom-made UPF1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
UPF1 Regulator of Nonsense Transcripts Homolog (UPF1) (AA 1-1129) protein (His tag)custom-made UPF1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
UPF1 Regulator of Nonsense Transcripts Homolog (UPF1) (AA 1019-1116) protein (GST tag)UPF1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- RENT1/UPF1 (UPF1) (UPF1 Regulator of Nonsense Transcripts Homolog (UPF1))
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Alternative Name
- Upf1,rent1
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Background
- This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants.
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Molecular Weight
- 122.9 kDa
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NCBI Accession
- NP_002902
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Pathways
- SARS-CoV-2 Protein Interactome
Target
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