UROS Protein (His tag)
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- Target See all UROS Proteins
- UROS (Uroporphyrinogen III Synthase (UROS))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This UROS protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human UROS protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- < 0.1 EU per μg protein as determined by LAL test
- Top Product
- Discover our top product UROS Protein
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Uroporphyrinogen III Synthase (UROS) (AA 1-265) protein (His tag)
UROS Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Uroporphyrinogen III Synthase (UROS) (AA 1-265) protein (GST tag)UROS Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Uroporphyrinogen III Synthase (UROS) (AA 1-265) protein (Strep Tag)Crystallography grade UROS Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Uroporphyrinogen III Synthase (UROS) (AA 1-265) protein (Strep Tag)Crystallography grade UROS Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Uroporphyrinogen III Synthase (UROS) protein (Myc-DYKDDDDK Tag)UROS Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 20 mM Tris-HCl, 100 mM NaCl, 10 % Glycerol, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- UROS (Uroporphyrinogen III Synthase (UROS))
- Alternative Name
- Uros (UROS Products)
- Synonyms
- wu:fd13d11 Protein, zgc:110240 Protein, UROIIIS Protein, Afu6g12800 Protein, UROS Protein, MGC85561 Protein, AI415298 Protein, Uros3 Protein, ARABIDOPSIS THALIANA UROPORPHYRINOGEN III SYNTHASE Protein, ATDUF3 Protein, ATUROS Protein, DOMAIN OF UNKNOWN FUNCTION 724 3 Protein, DUF3 Protein, T9J22.21 Protein, T9J22_21 Protein, UROPORPHYRINOGEN III SYNTHASE Protein, uroporphyrinogen III synthase Protein, uroporphyrinogen-III synthase (UroS) Protein, uroporphyrinogen-iii synthase Protein, uroporphyrinogen III synthase L homeolog Protein, uroporphyrinogen-III synthase family protein Protein, uros Protein, UROS Protein, AFUA_6G12800 Protein, UroS Protein, uros.L Protein, Uros Protein, HEMD Protein
- Background
- Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III, the branch point for the various sub-pathways leading to the wide diversity of porphyrins. Porphyrins act as cofactors for a multitude of enzymes that perform a variety of processes within the cell such as methionine synthesis (vitamin B12) or oxygen transport (heme). [UniProtKB/Swiss-Prot Function]
- Molecular Weight
- 29.7 kDa
- NCBI Accession
- NP_000366
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