VEGFB Protein
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- Target See all VEGFB Proteins
- VEGFB (Vascular Endothelial Growth Factor B (VEGFB))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human VEGF-B protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) in the presence of human VEGF 165. The expected ED50 for this effect is 1.0-2.0ug/mL.
- Top Product
- Discover our top product VEGFB Protein
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Vascular Endothelial Growth Factor B (VEGFB) (AA 1-207) protein (GST tag)
VEGFB Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Vascular Endothelial Growth Factor B (VEGFB) (AA 27-136) protein (GST tag)VEGFB Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Vascular Endothelial Growth Factor B (VEGFB) (Active) proteinVEGFB Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Vascular Endothelial Growth Factor B (VEGFB) (AA 22-207) protein (His tag)Crystallography grade VEGFB Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Vascular Endothelial Growth Factor B (VEGFB) (AA 22-207) (Active) protein (His tag)VEGFB Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- VEGFB (Vascular Endothelial Growth Factor B (VEGFB))
- Alternative Name
- Vegf-B (VEGFB Products)
- Synonyms
- VEGFL Protein, VRF Protein, VEGF-B Protein, Vrf Protein, vascular endothelial growth factor B Protein, VEGFB Protein, Vegfb Protein
- Background
- Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. [UniProtKB/Swiss-Prot Function]
- Molecular Weight
- 38 kDa
- NCBI Accession
- NP_003368
- Pathways
- RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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