VRK1 Protein (His tag)
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- Target See all VRK1 Proteins
- VRK1 (Vaccinia Related Kinase 1 (VRK1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This VRK1 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human VRK1 (full length, N-term HIS tag) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product VRK1 Protein
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Vaccinia Related Kinase 1 (VRK1) (AA 1-396) (Active) protein (GST tag)
VRK1 Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS, Func Active
Vaccinia Related Kinase 1 (VRK1) (AA 1-396) (Active) protein (His-GST)VRK1 Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
Vaccinia Related Kinase 1 (VRK1) (AA 1-396) protein (GST tag)VRK1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Vaccinia Related Kinase 1 (VRK1) (AA 287-396) protein (GST tag)VRK1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Vaccinia Related Kinase 1 (VRK1) (AA 1-396) protein (Strep Tag)Crystallography grade VRK1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- VRK1 (Vaccinia Related Kinase 1 (VRK1))
- Alternative Name
- Vrk1 (VRK1 Products)
- Synonyms
- vrk1 Protein, MGC64390 Protein, MGC75762 Protein, VRK1 Protein, PCH1 Protein, PCH1A Protein, 51PK Protein, wu:fe16f06 Protein, zgc:56266 Protein, vaccinia related kinase 1 L homeolog Protein, vaccinia related kinase 1 Protein, Serine/threonine-protein kinase VRK1 Protein, vrk1.L Protein, vrk1 Protein, VRK1 Protein, vrk-1 Protein, Vrk1 Protein
- Background
- This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 Molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.
- Molecular Weight
- 45.3 kDa
- NCBI Accession
- NP_003375
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