WNT1 Protein

Catalog No. ABIN2735607

Product Details

Target: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human WNT1 protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handling Advice: Resuspend the protein in the desired concentration in proper buffer

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Alternative Name: Wnt1 (WNT1 Products)

Synonyms: Xint-1 Protein, Xwnt1 Protein, int-1 Protein, int1 Protein, wnt-1 Protein, wnt1-a Protein, Int-1 Protein, Wnt-1 Protein, sw Protein, swaying Protein, BMND16 Protein, INT1 Protein, OI15 Protein, Int1 Protein, WNT-1 Protein, sb:eu647 Protein, zgc:194464 Protein, zgc:194478 Protein, WNT1 Protein, Wnt family member 1 Protein, Wnt family member 1 L homeolog Protein, wingless-type MMTV integration site family, member 1 Protein, WNT1 Protein, wnt1.L Protein, Wnt1 Protein, wnt1 Protein

Background: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98 % identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region.

Molecular Weight: 38.4 kDa

NCBI Accession: NP_005421

Pathways: WNT Signaling, Dopaminergic Neurogenesis