WT1 Protein (His tag)

Catalog No. ABIN2735633

Product Details

Target: WT1 (Wilms Tumor 1 (WT1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This WT1 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human WT1 / Wilms tumor protein (full length, N-term HIS tag, transcript variant A) protein expressed in E.coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: Wt1,wilms Tumor Protein (WT1 Products)

Synonyms: AWT1 Protein, EWS-WT1 Protein, GUD Protein, NPHS4 Protein, WAGR Protein, WIT-2 Protein, WT33 Protein, CWT1 Protein, WT1 Protein, XWT1a Protein, wt-1 Protein, wt1 Protein, xWT1 Protein, WT-1 Protein, gud Protein, wagr Protein, wt33 Protein, xwt1 Protein, wit-2 Protein, si:ch211-160j6.3 Protein, zgc:152697 Protein, D630046I19Rik Protein, Wt-1 Protein, XWT1b Protein, XeWT1 Protein, Wilms tumor 1 Protein, Wilms tumor 1 S homeolog Protein, wilms tumor 1b Protein, Wilms tumor protein Protein, Wilms tumor 1 homolog Protein, Wilms tumor 1 L homeolog Protein, WT1 Protein, wt1.S Protein, wt1 Protein, wt1b Protein, Wt1 Protein, wt1.L Protein

Background: This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.

Molecular Weight: 54.1 kDa

NCBI Accession: NP_000369

Pathways: Tube Formation