PATZ1 Protein (His tag)

Catalog No. ABIN2735981

Product Details

Target: PATZ1 (ZNF278) (Zinc Finger Protein 278 (ZNF278))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PATZ1 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human ZNF278 (full length, N-term HIS tag, transcript variant 4) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PATZ1 (ZNF278) (Zinc Finger Protein 278 (ZNF278))

Alternative Name: Znf278 (ZNF278 Products)

Synonyms: ZNF278 Protein, MAZR Protein, PATZ Protein, RIAZ Protein, ZBTB19 Protein, ZSG Protein, dJ400N23 Protein, 8430401L15Rik Protein, AI447519 Protein, Mazr Protein, Patz Protein, Zfp278 Protein, Znf278 Protein, POZ/BTB and AT hook containing zinc finger 1 Protein, POZ/BTB and AT hook containing zinc finger 1 S homeolog Protein, POZ (BTB) and AT hook containing zinc finger 1 Protein, POZ-, AT hook-, and zinc finger-containing protein 1 Protein, PATZ1 Protein, patz1.S Protein, Patz1 Protein, LOC100153931 Protein

Background: The protein encoded by this gene contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression, and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q, then the hybrid is thought to be translocated (t(122)(p36.1q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence containing the transactivation domain of EWS fused to zinc finger domain of this protein. This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Four alternatively spliced transcript variants are described for this gene.

Molecular Weight: 57.4 kDa

NCBI Accession: NP_114440