NADK Protein (AA 1-446) (His tag)
Quick Overview for NADK Protein (AA 1-446) (His tag) (ABIN2745746)
Target
See all NADK ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-446
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Purification tag / Conjugate
- This NADK protein is labelled with His tag.
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Purpose
- NAD Kinase (human) (rec.) (His) (highly active)
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Cross-Reactivity
- Human
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Characteristics
- Human full length NAD kinase (aa 1-446) is fused at the N-terminus to a His-tag.
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Endotoxin Level
- Free of any nucleotide converting side-activities.
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Biological Activity Comment
- >1U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1μmol of NADP per min.
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NAD Kinase (NADK) (AA 1-439) protein (His tag)
custom-made NADK Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
NAD Kinase (NADK) (AA 1-446) protein (His tag)custom-made NADK Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
NAD Kinase (NADK) (AA 64-298) protein (His tag)NADK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
NAD Kinase (NADK) (AA 1-110) protein (GST tag)NADK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
NAD Kinase (NADK) (AA 1-446) protein (GST tag)NADK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
- Prepare working aliquots by centrifugation and dissolve the pellet in 50 mM TRIS/HCl pH 7.5, 150 mM sodium chloride and 1 mM DTT.
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Concentration
- 1 mg/mL
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Buffer
- Ammonium sulphate suspension.
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Handling Advice
- After opening, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles.
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Storage
- 4 °C,-20 °C
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Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Working aliquots are stable for up to 3 months when stored at -20°C.
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- NADK (NAD Kinase (NADK))
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Alternative Name
- NAD Kinase
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Background
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NADK, Poly(P)/ATP NAD Kinase, EC 2.7.1.23
NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis.
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Molecular Weight
- ~50kDa (monomer)
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UniProt
- O95544
Target
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