IRF3 Protein (GST tag)
(4 references)-
- Target See all IRF3 Proteins
- IRF3 (Interferon Regulatory Factor 3 (IRF3))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This IRF3 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- IRF3 (Human) Recombinant Protein (P01)
- Sequence
- MGTPKPRILP WLVSQLDLGQ LEGVAWVNKS RTRFRIPWKH GLRQDAQQED FGIFQAWAEA TGAYVPGRDK PDLPTWKRNF RSALSRKEGL RLAEDRSKDP HDPHKIYEFV NSGVGDFSQP DTSPDTNGGG STSDTQEDIL DELLGNMVLA PLPDPGPPSL AVAPEPCPQP LRSPSLDNPT PFPNLGPSEN PLKRLLVPGE EWEFEVTAFY RGRQVFQQTI SCPEGLRLVG SEVGDRTLPG WPVTLPDPGM SLTDRGVMSY VRHVLSCLGG GLALWRAGQW LWAQRLGHCH TYWAVSEELL PNSGHGPDGE VPKGKEGGVF DLGPFIVGSW APRSDYLHGR KRTLTTLCPL VLCGGVMAPG PAVDQEARDG QGCAHVPQGL GRNGPGRGCL LPGEYCGPAH FQQPPTLPHL RPVQGLPAGL GGGHGFPGPW GDLSPRSSWC ASNPPVPHHL NQ
- Characteristics
- Human IRF3 full-length ORF ( AAH09395, 1 a.a. - 452 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Interferon Regulatory Factor 3 (IRF3) (AA 2-427) protein (His tag)
IRF3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interferon Regulatory Factor 3 (IRF3) (AA 1-427) protein (GST tag)IRF3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interferon Regulatory Factor 3 (IRF3) (AA 1-112) proteinIRF3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "The Golgi apparatus acts as a platform for TBK1 activation after viral RNA sensing." in: BMC biology, Vol. 14, pp. 69, (2016) (PubMed).
: "Quantitation of the dynamic profiles of the innate immune response using multiplex selected reaction monitoring-mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 6, pp. 1513-29, (2013) (PubMed).
: "NEMO binds ubiquitinated TANK-binding kinase 1 (TBK1) to regulate innate immune responses to RNA viruses." in: PLoS ONE, Vol. 7, Issue 9, pp. e43756, (2012) (PubMed).
: "Severe acute respiratory syndrome coronavirus M protein inhibits type I interferon production by impeding the formation of TRAF3.TANK.TBK1/IKKepsilon complex." in: The Journal of biological chemistry, Vol. 284, Issue 24, pp. 16202-9, (2009) (PubMed).
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: "The Golgi apparatus acts as a platform for TBK1 activation after viral RNA sensing." in: BMC biology, Vol. 14, pp. 69, (2016) (PubMed).
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- Target
- IRF3 (Interferon Regulatory Factor 3 (IRF3))
- Alternative Name
- IRF3 (IRF3 Products)
- Background
- Gene: interferon regulatory factor 3
- Gene ID
- 3661
- Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades
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