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FCGR3A Protein (AA 17-208) (His tag,AVI tag,Biotin)

FCGR3A Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Catalog No. ABIN2870546
  • Target See all FCGR3A Proteins
    FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 17-208
    Origin
    • 22
    • 3
    • 3
    • 1
    Human
    Source
    • 16
    • 6
    • 3
    • 2
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This FCGR3A protein is labelled with His tag,AVI tag,Biotin.
    Brand
    MABSol®,PrecisionAvi
    Sequence
    AA 17-208
    Specificity
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Characteristics
    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 25.5 kDa. The protein migrates as 40-55 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
    Purity
    >90 % as determined by SDS-PAGE.
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product FCGR3A Protein
  • Comment

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Handling Advice
    Please avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
  • Target
    FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))
    Alternative Name
    Fc gamma RIIIA / CD16a (FCGR3A Products)
    Synonyms
    CD16 Protein, CD16A Protein, FCG3 Protein, FCGR3 Protein, FCGRIII Protein, FCR-10 Protein, FCRIII Protein, FCRIIIA Protein, IGFR3 Protein, Fcgr4 Protein, Fc fragment of IgG receptor IIIa Protein, FCGR3A Protein, Fcgr3a Protein
    Background
    CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.
    Molecular Weight
    25.5 kDa
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