PDCD1LG2 Protein (AA 20-219) (Fc Tag,AVI tag,Biotin)

Catalog No. ABIN2870562

Product Details

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 20-219

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This PDCD1LG2 protein is labelled with Fc Tag,AVI tag,Biotin.

Brand: MABSol®,PrecisionAvi

Sequence: AA 20-219

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: This protein carries a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 51 kDa. The protein migrates as 50-55 kDa and 60-70 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Purity: >95 % as determined by reduced SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Alternative Name: PD-L2 (PDCD1LG2 Products)

Background: Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

Molecular Weight: 51.0 kDa