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PD-1 Protein (AA 25-167) (Fc Tag,AVI tag,Biotin)

PDCD1 Origin: Mouse Host: HEK-293 Cells Recombinant >90 % as determined by reduced SDS-PAGE. Active
Catalog No. ABIN2870576
  • Target See all PD-1 (PDCD1) Proteins
    PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 25-167
    Origin
    • 40
    • 14
    • 6
    • 4
    • 4
    • 3
    • 1
    Mouse
    Source
    • 52
    • 5
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This PD-1 protein is labelled with Fc Tag,AVI tag,Biotin.
    Brand
    MABSol®,PrecisionAvi
    Sequence
    AA 25-167
    Specificity
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Characteristics
    This protein carries a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 44.6 kDa. The protein migrates as 55-66 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
    Purity
    >90 % as determined by reduced SDS-PAGE.
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product PDCD1 Protein
  • Comment

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    Tris with Glycine, Arginine and NaCl,  pH 7.5
    Handling Advice
    Please avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
  • Target
    PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
    Alternative Name
    PD-1 (PDCD1 Products)
    Synonyms
    CD279 Protein, PD-1 Protein, PD1 Protein, SLEB2 Protein, hPD-1 Protein, hPD-l Protein, Ly101 Protein, Pdc1 Protein, programmed cell death 1 Protein, PDCD1 Protein, Pdcd1 Protein
    Background
    Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
    Molecular Weight
    44.6 kDa
    NCBI Accession
    NP_032824
    Pathways
    Cancer Immune Checkpoints
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