PD-L1 Protein (AA 19-238) (Fc Tag,AVI tag,Biotin)

Catalog No. ABIN2870578

Product Details

Target: PD-L1 (CD274 (PD-L1))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 19-238

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This PD-L1 protein is labelled with Fc Tag,AVI tag,Biotin.

Brand: MABSol®,PrecisionAvi

Sequence: AA 19-238

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: This protein carries a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 53.2 kDa. The protein migrates as 65-85 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: PD-L1 (PD-L1 Products)

Synonyms: B7-H Protein, B7H1 Protein, PD-L1 Protein, PDCD1L1 Protein, PDCD1LG1 Protein, PDL1 Protein, A530045L16Rik Protein, B7h1 Protein, Pdcd1l1 Protein, Pdcd1lg1 Protein, Pdl1 Protein, RGD1566211 Protein, CD274 molecule Protein, CD274 antigen Protein, programmed cell death 1 ligand 2 Protein, CD274 Protein, Cd274 Protein, PDCD1LG2 Protein

Background: Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.

Molecular Weight: 53.2 kDa

NCBI Accession: NP_068693

Pathways: Cancer Immune Checkpoints