CARS Protein (AA 2-748) (His tag)

Catalog No. ABIN3073487

Product Details

Target: CARS (Cysteinyl-tRNA Synthetase (CARS))

Protein Type: Recombinant

Protein Characteristics: AA 2-748

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This CARS protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: ADSSGQQGKG RRVQPQWSPP AGTQPCRLHL YNSLTRNKEV FIPQDGKKVT WYCCGPTVYD ASHMGHARSY ISFDILRRVL KDYFKFDVFY CMNITDIDDK IIKRARQNHL FEQYREKRPE AAQLLEDVQA ALKPFSVKLN ETTDPDKKQM LERIQHAVQL ATEPLEKAVQ SRLTGEEVNS CVEVLLEEAK DLLSDWLDST LGCDVTDNSI FSKLPKFWEG DFHRDMEALN VLPPDVLTRV SEYVPEIVNF VQKIVDNGYG YVSNGSVYFD TAKFASSEKH SYGKLVPEAV GDQKALQEGE GDLSISADRL SEKRSPNDFA LWKASKPGEP SWPCPWGKGR PGWHIECSAM AGTLLGASMD IHGGGFDLRF PHHDNELAQS EAYFENDCWV RYFLHTGHLT IAGCKMSKSL KNFITIKDAL KKHSARQLRL AFLMHSWKDT LDYSSNTMES ALQYEKFLNE FFLNVKDILR APVDITGQFE KWGEEEAELN KNFYDKKTAI HKALCDNVDT RTVMEEMRAL VSQCNLYMAA RKAVRKRPNQ ALLENIALYL THMLKIFGAV EEDSSLGFPV GGPGTSLSLE ATVMPYLQVL SEFREGVRKI AREQKVPEIL QLSDALRDNI LPELGVRFED HEGLPTVVKL VDRNTLLKER EEKRRVEEEK RKKKEEAARR KQEQEAAKLA KMKIPPSEMF LSETDKYSKF DENGLPTHDM EGKELSKGQA KKLKKLFEAQ EKLYKEYLQM AQNGSFQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human CARS Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CARS (Cysteinyl-tRNA Synthetase (CARS))

Alternative Name: CARS (CARS Products)

Synonyms: sb:cb71 Protein, CARS Protein, BcDNA:LD21177 Protein, CG8431 Protein, CRS Protein, CysRS Protein, Dmel\\CG8431 Protein, BA0089 Protein, DDBDRAFT_0215191 Protein, DDBDRAFT_0231320 Protein, DDB_0215191 Protein, DDB_0231320 Protein, DDBDRAFT_0187313 Protein, DDBDRAFT_0231318 Protein, DDB_0187313 Protein, DDB_0231318 Protein, T16B12.16 Protein, K15E6.3 Protein, K15E6_3 Protein, CARS1 Protein, CYSRS Protein, MGC:11246 Protein, CA3 Protein, cysteinyl-tRNA synthetase Protein, Cysteinyl-tRNA synthetase Protein, cysteine--tRNA ligase Protein, cysteine-tRNA ligase Protein, Cysteinyl-tRNA synthetase, class Ia family protein Protein, cysteinyl-tRNA synthetase CysS Protein, cysteinyl-tRNA synthetase S homeolog Protein, CARS Protein, cars Protein, CysRS Protein, cysS Protein, APH_RS02395 Protein, mcysS Protein, SYCO ARATH Protein, AT3G56300 Protein, AT5G38830 Protein, cars.S Protein, Cars Protein

Molecular Weight: 86.3 kDa Including tag.

UniProt: P49589