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TFB2M Protein (AA 20-396) (His tag)

Crystallography grade TFB2M Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Catalog No. ABIN3073837
  • Target See all TFB2M Proteins
    TFB2M (Transcription Factor B2, Mitochondrial (TFB2M))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 20-396
    Origin
    • 5
    • 1
    • 1
    Human
    Source
    • 2
    • 2
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This TFB2M protein is labelled with His tag.
    Application
    ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
    Sequence
    AGRFCILGSE AATRKHLPAR NHCGLSDSSP QLWPEPDFRN PPRKASKASL DFKRYVTDRR LAETLAQIYL GKPSRPPHLL LECNPGPGIL TQALLEAGAK VVALESDKTF IPHLESLGKN LDGKLRVIHC DFFKLDPRSG GVIKPPAMSS RGLFKNLGIE AVPWTADIPL KVVGMFPSRG EKRALWKLAY DLYSCTSIYK FGRIEVNMFI GEKEFQKLMA DPGNPDLYHV LSVIWQLACE IKVLHMEPWS SFDIYTRKGP LENPKRRELL DQLQQKLYLI QMIPRQNLFT KNLTPMNYNI FFHLLKHCFG RRSATVIDHL RSLTPLDARD ILMQIGKQED EKVVNMHPQD FKTLFETIER SKDCAYKWLY DETLEDR
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human TFB2M Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product TFB2M Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    TFB2M (Transcription Factor B2, Mitochondrial (TFB2M))
    Alternative Name
    TFB2M (TFB2M Products)
    Synonyms
    CG3910 Protein, Dmel\\CG3910 Protein, TFB2 Protein, d-TF2BM Protein, d-mtTFB2 Protein, TFB2M Protein, sb:cb857 Protein, hkp1 Protein, Hkp1 Protein, mtTFB2 Protein, mitochondrial transcription factor B2 Protein, transcription factor B2, mitochondrial Protein, mtTFB2 Protein, TFB2M Protein, tfb2m Protein, Tfb2m Protein
    Background
    S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity. {ECO:0000269|PubMed:12068295, ECO:0000269|PubMed:12897151, ECO:0000269|PubMed:15526033, ECO:0000269|PubMed:20410300}.
    Molecular Weight
    44.2 kDa Including tag.
    UniProt
    Q9H5Q4
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