CCT4 Protein (AA 2-539) (His tag)

Catalog No. ABIN3074016

Product Details

Target: CCT4 (Chaperonin Containing TCP1, Subunit 4 (Delta) (CCT4))

Protein Type: Recombinant

Protein Characteristics: AA 2-539

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This CCT4 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: PENVAPRSGA TAGAAGGRGK GAYQDRDKPA QIRFSNISAA KAVADAIRTS LGPKGMDKMI QDGKGDVTIT NDGATILKQM QVLHPAARML VELSKAQDIE AGDGTTSVVI IAGSLLDSCT KLLQKGIHPT IISESFQKAL EKGIEILTDM SRPVELSDRE TLLNSATTSL NSKVVSQYSS LLSPMSVNAV MKVIDPATAT SVDLRDIKIV KKLGGTIDDC ELVEGLVLTQ KVSNSGITRV EKAKIGLIQF CLSAPKTDMD NQIVVSDYAQ MDRVLREERA YILNLVKQIK KTGCNVLLIQ KSILRDALSD LALHFLNKMK IMVIKDIERE DIEFICKTIG TKPVAHIDQF TADMLGSAEL AEEVNLNGSG KLLKITGCAS PGKTVTIVVR GSNKLVIEEA ERSIHDALCV IRCLVKKRAL IAGGGAPEIE LALRLTEYSR TLSGMESYCV RAFADAMEVI PSTLAENAGL NPISTVTELR NRHAQGEKTA GINVRKGGIS NILEELVVQP LLVSVSALTL ATETVRSILK IDDVVNTR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human CCT4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CCT4 (Chaperonin Containing TCP1, Subunit 4 (Delta) (CCT4))

Alternative Name: CCT4 (CCT4 Products)

Synonyms: zgc:65789 Protein, zgc:77192 Protein, Cct4 Protein, Dmel\\CG5525 Protein, Tcp-1delta Protein, Tcp1-delta Protein, cct4 Protein, MGC89275 Protein, CCT4 Protein, DKFZp459D1424 Protein, LOC100225853 Protein, cctd Protein, CCT-DELTA Protein, Cctd Protein, SRB Protein, 2610204B21Rik Protein, A45 Protein, C78323 Protein, chaperonin containing TCP1, subunit 4 (delta) Protein, chaperonin containing TCP1 subunit 4 Protein, chaperonin containing TCP1 subunit 4 L homeolog Protein, CG5525 gene product from transcript CG5525-RA Protein, chaperonin containing Tcp1, subunit 4 (delta) Protein, T-complex protein 1 subunit delta Protein, cct4 Protein, CCT4 Protein, cct4.L Protein, CG5525 Protein, Cct4 Protein, cct-4 Protein

Background: Molecular chaperone, assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. {ECO:0000269|PubMed:20080638}.

Molecular Weight: 58.7 kDa Including tag.

UniProt: P50991

Pathways: Embryonic Body Morphogenesis