TIMM50 Protein (AA 87-353) (His tag)

Catalog No. ABIN3074044

Product Details

Target: TIMM50 (Translocase of Inner Mitochondrial Membrane 50 Homolog (TIMM50))

Protein Type: Recombinant

Protein Characteristics: AA 87-353

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This TIMM50 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: GNNPVDENGA KIPDEFDNDP ILVQQLRRTY KYFKDYRQMI IEPTSPCLLP DPLQEPYYQP PYTLVLELTG VLLHPEWSLA TGWRFKKRPG IETLFQQLAP LYEIVIFTSE TGMTAFPLID SVDPHGFISY RLFRDATRYM DGHHVKDISC LNRDPARVVV VDCKKEAFRL QPYNGVALRP WDGNSDDRVL LDLSAFLKTI ALNGVEDVRT VLEHYALEDD PLAAFKQRQS RLEQEEQQRL AELSKSNKQN LFLGSLTSRL WPRSKQP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human TIMM50 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: TIMM50 (Translocase of Inner Mitochondrial Membrane 50 Homolog (TIMM50))

Alternative Name: TIMM50 (TIMM50 Products)

Synonyms: F14J16.15 Protein, F14J16_15 Protein, emb1860 Protein, embryo defective 1860 Protein, 2810403L02Rik Protein, AU015082 Protein, TIM50L Protein, TIM50 Protein, Haloacid dehalogenase-like hydrolase (HAD) superfamily protein Protein, protein translocase subunit Protein, translocase of inner mitochondrial membrane 50 Protein, TIM50 Protein, Timm50 Protein, TIMM50 Protein

Background: Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro, however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body. {ECO:0000269|PubMed:15044455}.

Molecular Weight: 31.8 kDa Including tag.

UniProt: Q3ZCQ8