TIMM8A/DDP Protein (AA 1-97) (His tag)
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- Target See all TIMM8A/DDP (TIMM8A) Proteins
- TIMM8A/DDP (TIMM8A) (Translocase of Inner Mitochondrial Membrane 8A (TIMM8A))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-97
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This TIMM8A/DDP protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- Sequence
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MDSSSSSSAA GLGAVDPQLQ HFIEVETQKQ RFQQLVHQMT ELCWEKCMDK PGPKLDSRAE ACFVNCVERF IDTSQFILNR LEQTQKSKPV FSESLSD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human TIMM8A Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Grade
- Crystallography grade
- Top Product
- Discover our top product TIMM8A Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- TIMM8A/DDP (TIMM8A) (Translocase of Inner Mitochondrial Membrane 8A (TIMM8A))
- Alternative Name
- TIMM8A (TIMM8A Products)
- Synonyms
- TIMM8A Protein, ddp Protein, tim8a Protein, timm8a Protein, DDP Protein, DDP1 Protein, DFN1 Protein, MTS Protein, TIM8 Protein, im:6896085 Protein, zgc:100916 Protein, Ddp1 Protein, Timm8a Protein, DXHXS1274E Protein, Fci-12 Protein, Tim8a Protein, translocase of inner mitochondrial membrane 8 homolog A (yeast) Protein, translocase of inner mitochondrial membrane 8A Protein, translocase of inner mitochondrial membrane 8 homolog A1 (yeast) Protein, translocase of inner mitochondrial membrane 8A1 Protein, TIMM8A Protein, timm8a Protein, Timm8a1 Protein
- Background
- Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins. Probably necessary for normal neurologic development. {ECO:0000269|PubMed:11489896, ECO:0000269|PubMed:15254020}.
- Molecular Weight
- 12.0 kDa Including tag.
- UniProt
- O60220
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