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VTA1 Protein (AA 2-307) (His tag)

Crystallography grade VTA1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, Crys, WB
Catalog No. ABIN3075432
  • Target See all VTA1 Proteins
    VTA1 (Vps20-Associated 1 Homolog (VTA1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-307
    Origin
    • 2
    • 1
    • 1
    Human
    Source
    • 2
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This VTA1 protein is labelled with His tag.
    Application
    SDS-PAGE (SDS), ELISA, Crystallization (Crys), Western Blotting (WB)
    Sequence
    AALAPLPPLP AQFKSIQHHL RTAQEHDKRD PVVAYYCRLY AMQTGMKIDS KTPECRKFLS KLMDQLEALK KQLGDNEAIT QEIVGCAHLE NYALKMFLYA DNEDRAGRFH KNMIKSFYTA SLLIDVITVF GELTDENVKH RKYARWKATY IHNCLKNGET PQAGPVGIEE DNDIEENEDA GAASLPTQPT QPSSSSTYDP SNMPSGNYTG IQIPPGAHAP ANTPAEVPHS TGVASNTIQP TPQTIPAIDP ALFNTISQGD VRLTPEDFAR AQKYCKYAGS ALQYEDVSTA VQNLQKALKL LTTGRE
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human VTA1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product VTA1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    VTA1 (Vps20-Associated 1 Homolog (VTA1))
    Alternative Name
    VTA1 (VTA1 Products)
    Synonyms
    C6orf55 Protein, DRG-1 Protein, DRG1 Protein, LIP5 Protein, My012 Protein, SBP1 Protein, 1110001D18Rik Protein, 1110059P08Rik Protein, AU040813 Protein, C85340 Protein, Lip5 Protein, RGD1305031 Protein, Vps20-associated 1 homolog Protein, vesicle trafficking 1 Protein, vesicle (multivesicular body) trafficking 1 Protein, Vta1 Protein, VTA1 Protein
    Background
    Involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. Involved in the sorting and down-regulation of EGFR (By similarity). Involved in HIV-1 budding. {ECO:0000250, ECO:0000269|PubMed:15644320}.
    Molecular Weight
    34.7 kDa Including tag.
    UniProt
    Q9NP79
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