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WASP Protein (AA 2-502) (His tag)

Crystallography grade WAS Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3075435
  • Target See all WASP (WAS) Proteins
    WASP (WAS) (Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-502
    Origin
    • 4
    • 1
    Human
    Source
    • 2
    • 2
    • 1
    Insect Cells
    Purification tag / Conjugate
    This WASP protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    SGGPMGGRPG GRGAPAVQQN IPSTLLQDHE NQRLFEMLGR KCLTLATAVV QLYLALPPGA EHWTKEHCGA VCFVKDNPQK SYFIRLYGLQ AGRLLWEQEL YSQLVYSTPT PFFHTFAGDD CQAGLNFADE DEAQAFRALV QEKIQKRNQR QSGDRRQLPP PPTPANEERR GGLPPLPLHP GGDQGGPPVG PLSLGLATVD IQNPDITSSR YRGLPAPGPS PADKKRSGKK KISKADIGAP SGFKHVSHVG WDPQNGFDVN NLDPDLRSLF SRAGISEAQL TDAETSKLIY DFIEDQGGLE AVRQEMRRQE PLPPPPPPSR GGNQLPRPPI VGGNKGRSGP LPPVPLGIAP PPPTPRGPPP PGRGGPPPPP PPATGRSGPL PPPPPGAGGP PMPPPPPPPP PPPSSGNGPA PPPLPPALVP AGGLAPGGGR GALLDQIRQG IQLNKTPGAP ESSALQPPPQ SSEGLVGALM HVMQKRSRAI HSSDEGEDQA GDEDEDDEWD D
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human WAS Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product WAS Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    WASP (WAS) (Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS))
    Alternative Name
    WAS (WAS Products)
    Synonyms
    IMD2 Protein, SCNX Protein, THC Protein, THC1 Protein, WASP Protein, U42471 Protein, WASp Protein, Wasp Protein, imd2 Protein, thc Protein, thc1 Protein, was Protein, wasp Protein, wu:fk81c08 Protein, zgc:64164 Protein, Wiskott-Aldrich syndrome Protein, neural Wiskott-Aldrich syndrome protein Protein, wiskott-aldrich syndrome protein Protein, Wiskott-Aldrich syndrome L homeolog Protein, Wiskott-Aldrich syndrome (eczema-thrombocytopenia) b Protein, WAS Protein, LOC5578888 Protein, CpipJ_CPIJ006699 Protein, Was Protein, was.L Protein, wasb Protein
    Background
    Effector protein for Rho-type GTPases. Regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. {ECO:0000269|PubMed:12235133, ECO:0000269|PubMed:16275905, ECO:0000269|PubMed:18650809}.
    Molecular Weight
    53.7 kDa Including tag.
    UniProt
    P42768
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