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SARDH Protein (AA 23-918) (His tag)

Crystallography grade SARDH Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3076575
$24,004.89
Plus shipping costs $50.00, dry ice fee $20.00 and VAT
Shipping to: United States
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  • Target See all SARDH Proteins
    SARDH (Sarcosine Dehydrogenase (SARDH))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 23-918
    Origin
    • 3
    • 1
    Human
    Source
    • 1
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This SARDH protein is labelled with His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MGPCNLSSAA GPTAEKSVPY QRTLKEGQGT SVVAQGPSRP LPSTANVVVI GGGSLGCQTL YHLAKLGMSG AVLLERERLT SGTTWHTAGL LWQLRPSDVE VELLAHTRRV VSRELEEETG LHTGWIQNGG LFIASNRQRL DEYKRLMSLG KAYGVESHVL SPAETKTLYP LMNVDDLYGT LYVPHDGTMD PAGTCTTLAR AASARGAQVI ENCPVTGIRV WTDDFGVRRV AGVETQHGSI QTPCVVNCAG VWASAVGRMA GVKVPLVAMH HAYVVTERIE GIQNMPNVRD HDASVYLRLQ GDALSVGGYE ANPIFWEEVS DKFAFGLFDL DWEVFTQHIE GAINRVPVLE KTGIKSTVCG PESFTPDHKP LMGEAPELRG FFLGCGFNSA GMMLGGGCGQ ELAHWIIHGR PEKDMHGYDI RRFHHSLTDH PRWIRERSHE SYAKNYSVVF PHDEPLAGRN MRRDPLHEEL LGQGCVFQER HGWERPGWFH PRGPAPVLEY DYYGAYGSRA HEDYAYRRLL ADEYTFAFPP HHDTIKKECL ACRGAAAVFD MSYFGKFYLV GLDARKAADW LFSADVSRPP GSTVYTCMLN HRGGTESDLT VSRLAPSHQA SPLAPAFEGD GYYLAMGGAV AQHNWSHITT VLQDQKSQCQ LIDSSEDLGM ISIQGPASRA ILQEVLDADL SNEAFPFSTH KLLRAAGHLV RAMRLSFVGE LGWELHIPKA SCVPVYRAVM AAGAKHGLIN AGYRAIDSLS IEKGYRHWHA DLRPDDSPLE AGLAFTCKLK SPVPFLGREA LEQQRAAGLR RRLVCFTMED KVPMFGLEAI WRNGQVVGHV RRADFGFAID KTIAYGYIHD PSGGPVSLDF VKSGDYALER MGVTYGAQAH LKSPFDPNNK RVKGIY
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human SARDH Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    SARDH (Sarcosine Dehydrogenase (SARDH))
    Alternative Name
    SARDH (SARDH Products)
    Synonyms
    BPR-2 Protein, DMGDHL1 Protein, SAR Protein, SARD Protein, SDH Protein, zgc:56363 Protein, sarcosine dehydrogenase Protein, sarcosine dehydrogenase, mitochondrial Protein, Sarcosine dehydrogenase Protein, SARDH Protein, SAV_6951 Protein, LOC5565677 Protein, LOC5572760 Protein, SDH Protein, NGR_b16520 Protein, Sinme_2254 Protein, sardh Protein, Sardh Protein
    Molecular Weight
    99.7 kDa Including tag.
    UniProt
    Q9UL12
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