OXCT1 Protein (AA 40-520) (His tag)

Catalog No. ABIN3076587

Product Details

Target: OXCT1 (3-Oxoacid CoA Transferase 1 (OXCT1))

Protein Type: Recombinant

Protein Characteristics: AA 40-520

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This OXCT1 protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: TKFYTDPVEA VKDIPDGATV LVGGFGLCGI PENLIDALLK TGVKGLTAVS NNAGVDNFGL GLLLRSKQIK RMVSSYVGEN AEFERQYLSG ELEVELTPQG TLAERIRAGG AGVPAFYTPT GYGTLVQEGG SPIKYNKDGS VAIASKPREV REFNGQHFIL EEAITGDFAL VKAWKADRAG NVIFRKSARN FNLPMCKAAE TTVVEVEEIV DIGAFAPEDI HIPQIYVHRL IKGEKYEKRI ERLSIRKEGD GEAKSAKPGD DVRERIIKRA ALEFEDGMYA NLGIGIPLLA SNFISPNITV HLQSENGVLG LGPYPRQHEA DADLINAGKE TVTILPGASF FSSDESFAMI RGGHVDLTML GAMQVSKYGD LANWMIPGKM VKGMGGAMDL VSSAKTKVVV TMEHSAKGNA HKIMEKCTLP LTGKQCVNRI ITEKAVFDVD KKKGLTLIEL WEGLTVDDVQ KSTGCDFAVS PKLMPMQQIA N
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human OXCT1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: OXCT1 (3-Oxoacid CoA Transferase 1 (OXCT1))

Alternative Name: OXCT1 (OXCT1 Products)

Synonyms: OXCT Protein, oxct1 Protein, zgc:92003 Protein, wu:fj36f06 Protein, wu:fj48g08 Protein, OXCT1 Protein, oxct Protein, scot Protein, SCOT Protein, 2610008O03Rik Protein, Oxct Protein, Oxct2a Protein, Scot-s Protein, 3-oxoacid CoA-transferase 1 Protein, 3-oxoacid CoA transferase 1a Protein, 3-oxoacid CoA transferase 1 Protein, 3-oxoacid CoA-transferase 1 L homeolog Protein, succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial Protein, OXCT1 Protein, oxct1a Protein, oxct1 Protein, Oxct1 Protein, oxct1.L Protein, MONBRDRAFT_35053 Protein, LOC100414476 Protein

Background: Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.

Molecular Weight: 53.0 kDa Including tag.

UniProt: P55809

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis