Syntaxin 5 Protein (STX5) (AA 1-333) (His tag)

Catalog No. ABIN3077517

Product Details

Target: Syntaxin 5 (STX5)

Protein Type: Recombinant

Protein Characteristics: AA 1-333

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Syntaxin 5 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: MIPRKRYGSK NTDQGVYLGL SKTQVLSPAT AGSSSSDIAP LPPPVTLVPP PPDTMSCRDR TQEFLSACKS LQTRQNGIQT NKPALRAVRQ RSEFTLMAKR IGKDLSNTFA KLEKLTILAK RKSLFDDKAV EIEELTYIIK QDINSLNKQI AQLQDFVRAK GSQSGRHLQT HSNTIVVSLQ SKLASMSNDF KSVLEVRTEN LKQQRSRREQ FSRAPVSALP LAPNHLGGGA VVLGAESHAS KDVAIDMMDS RTSQQLQLID EQDSYIQSRA DTMQNIESTI VELGSIFQQL AHMVKEQEET IQRIDENVLG AQLDVEAAHS EILKYFQSVT SNR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human STX5 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Syntaxin 5 (STX5)

Alternative Name: STX5 (STX5 Products)

Synonyms: BG:DS02740.12 Protein, CG4214 Protein, DmSyx5 Protein, Dmel\\CG4214 Protein, II Protein, SED5 Protein, SYN5 Protein, Sed5 Protein, Synx5 Protein, Syx Protein, Syx5A Protein, anon-35Fb Protein, anon-35Fd Protein, dSed5 Protein, dSed5p Protein, dSyx5 Protein, dsed5 Protein, l(2)35Ff Protein, sed5 Protein, syn5 Protein, syx5 Protein, zgc:91864 Protein, wu:fj81f08 Protein, stx5a Protein, Syntaxin-5 Protein, MGC114979 Protein, STX5A Protein, Stx5a Protein, 0610031F24Rik Protein, D19Ertd627e Protein, Stx5 Protein, Syntaxin 5 Protein, syntaxin 5A Protein, syntaxin 5 Protein, syntaxin 5 L homeolog Protein, syntaxin 5, putative Protein, Qa-SNARE protein Protein, Syx5 Protein, stx5a Protein, STX5 Protein, stx5 Protein, stx5.L Protein, TA12540 Protein, LMJF_32_0070 Protein, LOC732925 Protein, Stx5 Protein, Stx5a Protein

Background: Mediates endoplasmic reticulum to Golgi transport. Together with p115/USO1 and GM130/GOLGA2, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. {ECO:0000250|UniProtKB:Q08851}.

Molecular Weight: 38.0 kDa Including tag.

UniProt: Q13190

Pathways: Transition Metal Ion Homeostasis, Regulation of Cell Size, Synaptic Vesicle Exocytosis