DCMP Deaminase (DCTD) (AA 1-178) protein (His tag)
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- Target See all DCMP Deaminase (DCTD) Proteins
- DCMP Deaminase (DCTD)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-178
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Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- His tag
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- Sequence
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MSEVSCKKRD DYLEWPEYFM AVAFLSAQRS KDPNSQVGAC IVNSENKIVG IGYNGMPNGC SDDVLPWRRT AENKLDTKYP YVCHAELNAI MNKNSTDVKG CSMYVALFPC NECAKLIIQA GIKEVIFMSD KYHDSDEATA ARLLFNMAGV TFRKFIPKCS KIVIDFDSIN SRPSQKLQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human DCTD Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product DCTD Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- DCMP Deaminase (DCTD)
- Alternative Name
- DCTD (DCTD Products)
- Synonyms
- zgc:110226 Protein, DCTD Protein, 6030466N05Rik Protein, Afu2g06240 Protein, MGC81193 Protein, dCMP deaminase Protein, deoxycytidylate deaminase Protein, cell division protein DedD Protein, dCMP deaminase L homeolog Protein, conserved uncharacterised protein Protein, DCTD Protein, dctd Protein, dctD Protein, Dctd Protein, AFUA_2G06240 Protein, ACLA_068070 Protein, LELG_03636 Protein, VIBHAR_RS20285 Protein, LOC5564022 Protein, Ccur_00700 Protein, PAAG_00286 Protein, SPAP_0719 Protein, LOC100285892 Protein, dctd.L Protein, CNB00950 Protein, THAPS_42740 Protein, dCMP deaminase Protein, Thena_0587 Protein, Ccan_15560 Protein
- Target Type
- Viral Protein
- Background
- Supplies the nucleotide substrate for thymidylate synthetase.
- Molecular Weight
- 21.0 kDa Including tag.
- UniProt
- P32321
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