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Desmocollin 3 Protein (DSC3) (AA 712-896) (His tag)

Crystallography grade DSC3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3079072
  • Target See all Desmocollin 3 (DSC3) Proteins
    Desmocollin 3 (DSC3)
    Protein Type
    Recombinant
    Protein Characteristics
    AA 712-896
    Origin
    • 4
    • 4
    • 1
    Human
    Source
    • 5
    • 3
    • 1
    Insect Cells
    Purification tag / Conjugate
    This Desmocollin 3 protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    MCGVFGATKG KRFPEDLAQQ NLIISNTEAP GDDRVCSANG FMTQTTNNSS QGFCGTMGSG M KNGGQETIEM MKGGNQTLES CRGAGHHHTL DSCRGGHTEV DNCRYTYSEW HSFTQPRLGE KLHRCNQNED RMPSQDYVLT YNYEGRGSPA GSVGCCSEKQ EEDGLDFLNN LEPKFITLAE ACTKRHHHHH H
    Sequence includes C-terminal His-tag
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PLP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    The protein is purified from the cleared cell lysate using His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    In solution (20 mM Hepes, pH 7.4, 100 mM NaCl,)
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Chernyavsky, Amber, Agnoletti, Wang, Grando: "Synergy among non-desmoglein antibodies contributes to the immunopathology of desmoglein antibody-negative pemphigus vulgaris." in: The Journal of biological chemistry, Vol. 294, Issue 12, pp. 4520-4528, (2019) (PubMed).

  • Target
    Desmocollin 3 (DSC3)
    Alternative Name
    DSC3 (DSC3 Products)
    Synonyms
    CDHF3 Protein, DSC Protein, DSC1 Protein, DSC2 Protein, DSC4 Protein, HT-CP Protein, 5430426I24Rik Protein, MGC85083 Protein, desmocollin 3 Protein, desmocollin 3 S homeolog Protein, DSC3 Protein, Dsc3 Protein, dsc3.S Protein, dsc3 Protein
    Background
    Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms.
    Molecular Weight
    21.3 kDa Including tag
    UniProt
    Q14574
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