EIF3K Protein (AA 2-218) (His tag)

Catalog No. ABIN3079326

Product Details

Target: EIF3K (Eukaryotic Translation Initiation Factor 3 Subunit K (EIF3K))

Protein Type: Recombinant

Protein Characteristics: AA 2-218

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This EIF3K protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: AMFEQMRANV GKLLKGIDRY NPENLATLER YVETQAKENA YDLEANLAVL KLYQFNPAFF QTTVTAQILL KALTNLPHTD FTLCKCMIDQ AHQEERPIRQ ILYLGDLLET CHFQAFWQAL DENMDLLEGI TGFEDSVRKF ICHVVGITYQ HIDRWLLAEM LGDLSDSQLK VWMSKYGWSA DESGQIFICS QEESIKPKNI VEKIDFDSVS SIMASSQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human EIF3K Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: EIF3K (Eukaryotic Translation Initiation Factor 3 Subunit K (EIF3K))

Alternative Name: EIF3K (EIF3K Products)

Synonyms: eif3s12 Protein, EIF3S12 Protein, ACYPI000023 Protein, eIF3k Protein, EIF3-p28 Protein, HSPC029 Protein, M9 Protein, MSTP001 Protein, PLAC-24 Protein, PLAC24 Protein, PRO1474 Protein, PTD001 Protein, 1200009C21Rik Protein, Eif3s12 Protein, zgc:110726 Protein, eukaryotic translation initiation factor 3 subunit K L homeolog Protein, eukaryotic translation initiation factor 3 subunit K Protein, eukaryotic translation initiation factor 3, subunit K Protein, eukaryotic translation initiation factor 3 subunit k Protein, Eukaryotic translation initiation factor 3 subunit K Protein, eif3k.L Protein, EIF3K Protein, eif3k Protein, ANI_1_1610144 Protein, AOR_1_2266174 Protein, Tsp_00068 Protein, Tsp_13184 Protein, Eif3k Protein, LOC733122 Protein, eif-3.K Protein

Background: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Molecular Weight: 25.9 kDa Including tag.

UniProt: Q9UBQ5

Pathways: Ribonucleoprotein Complex Subunit Organization