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FCRL5 Protein (AA 16-851) (His tag)

Crystallography grade FCRL5 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, Crys, WB
Catalog No. ABIN3079958
  • Target See all FCRL5 Proteins
    FCRL5 (Fc Receptor-Like 5 (FCRL5))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 16-851
    Origin
    • 5
    • 2
    Human
    Source
    • 4
    • 3
    Insect Cells
    Purification tag / Conjugate
    This FCRL5 protein is labelled with His tag.
    Application
    ELISA, SDS-PAGE (SDS), Crystallization (Crys), Western Blotting (WB)
    Sequence
    QFARTPRPII FLQPPWTTVF QGERVTLTCK GFRFYSPQKT KWYHRYLGKE ILRETPDNIL EVQESGEYRC QAQGSPLSSP VHLDFSSASL ILQAPLSVFE GDSVVLRCRA KAEVTLNNTI YKNDNVLAFL NKRTDFHIPH ACLKDNGAYR CTGYKESCCP VSSNTVKIQV QEPFTRPVLR ASSFQPISGN PVTLTCETQL SLERSDVPLR FRFFRDDQTL GLGWSLSPNF QITAMWSKDS GFYWCKAATM PYSVISDSPR SWIQVQIPAS HPVLTLSPEK ALNFEGTKVT LHCETQEDSL RTLYRFYHEG VPLRHKSVRC ERGASISFSL TTENSGNYYC TADNGLGAKP SKAVSLSVTV PVSHPVLNLS SPEDLIFEGA KVTLHCEAQR GSLPILYQFH HEGAALERRS ANSAGGVAIS FSLTAEHSGN YYCTADNGFG PQRSKAVSLS VTVPVSHPVL TLSSAEALTF EGATVTLHCE VQRGSPQILY QFYHEDMPLW SSSTPSVGRV SFSFSLTEGH SGNYYCTADN GFGPQRSEVV SLFVTVPVSR PILTLRVPRA QAVVGDLLEL HCEAPRGSPP ILYWFYHEDV TLGSSSAPSG GEASFNLSLT AEHSGNYSCE ANNGLVAQHS DTISLSVIVP VSRPILTFRA PRAQAVVGDL LELHCEALRG SSPILYWFYH EDVTLGKISA PSGGGASFNL SLTTEHSGIY SCEADNGLEA QRSEMVTLKV AVPVSRPVLT LRAPGTHAAV GDLLELHCEA LRGSPLILYR FFHEDVTLGN RSSPSGGASL NLSLTAEHSG NYSCEADNGL GAQRSETVTL YITGLTANRS GPFATG
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human FCRL5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product FCRL5 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    FCRL5 (Fc Receptor-Like 5 (FCRL5))
    Alternative Name
    FCRL5 (FCRL5 Products)
    Synonyms
    FCRL5 Protein, BXMAS1 Protein, CD307 Protein, CD307e Protein, FCRH5 Protein, IRTA2 Protein, PRO820 Protein, Fcrh3 Protein, mBXMH2 Protein, Fc receptor like 5 Protein, Fc receptor-like 5 Protein, FCRL5 Protein, Fcrl5 Protein
    Background
    May be involved in B-cell development and differentiation in peripheral lymphoid organs and may be useful markers of B-cell stages. May have an immunoregulatory role in marginal zone B-cells. {ECO:0000269|PubMed:11453668}.
    Molecular Weight
    92.2 kDa Including tag.
    UniProt
    Q96RD9
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