FZD5 Protein (AA 27-238) (His tag)

Catalog No. ABIN3080340

Product Details

Target: FZD5 (Frizzled Family Receptor 5 (FZD5))

Protein Type: Recombinant

Protein Characteristics: AA 27-238

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This FZD5 protein is labelled with His tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)

Sequence: ASKAPVCQEI TVPMCRGIGY NLTHMPNQFN HDTQDEAGLE VHQFWPLVEI QCSPDLRFFL CSMYTPICLP DYHKPLPPCR SVCERAKAGC SPLMRQYGFA WPERMSCDRL PVLGRDAEVL CMDYNRSEAT TAPPRPFPAK PTLPGPPGAP ASGGECPAGG PFVCKCREPF VPILKESHPL YNKVRTGQVP NCAVPCYQPS FSADERTFAT FW
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human FZD5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: FZD5 (Frizzled Family Receptor 5 (FZD5))

Alternative Name: FZD5 (FZD5 Products)

Synonyms: C2orf31 Protein, HFZ5 Protein, 5330434N09Rik Protein, AI427138 Protein, Fz-5 Protein, Fz5 Protein, mFz5 Protein, Xfz5 Protein, frizzled5 Protein, frizzled5a Protein, fz5 Protein, fzd5-A Protein, fz2 Protein, fz8c Protein, fzd8c Protein, zg02 Protein, frizzled class receptor 5 Protein, frizzled class receptor 5 S homeolog Protein, FZD5 Protein, Fzd5 Protein, fzd5.S Protein, fzd5 Protein

Background: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta-catenin pathway. {ECO:0000269|PubMed:10097073}.

Molecular Weight: 24.5 kDa Including tag.

UniProt: Q13467

Pathways: WNT Signaling