Glucose-6-Phosphatase, Catalytic, 2 (G6PC2) (AA 1-355) protein (rho-1D4 tag)

Details for Product No. ABIN3080375, Supplier: Log in to see
Protein Name
  • IGRP
  • G6pc-rs
  • Igrp
  • g6pc2
  • igrp
  • glucose-6-phosphatase catalytic subunit 2
  • glucose-6-phosphatase, catalytic, 2
  • glucose-6-phosphatase, catalytic, 2 S homeolog
  • G6PC2
  • G6pc2
  • g6pc2.S
Protein Characteristics
AA 1-355
1
Origin
Human
1
1
Source
Insect Cells
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This G6PC2 protein is labelled with rho-1D4 tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
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Sequence MDFLHRNGVL IIQHLQKDYR AYYTFLNFMS NVGDPRNIFF IYFPLCFQFN QTVGTKMIWV AVIGDWLNLI FKWILFGHRP YWWVQETQIY PNHSSPCLEQ FPTTCETGPG SPSGHAMGAS CVWYVMVTAA LSHTVCGMDK FSITLHRLTW SFLWSVFWLI QISVCISRVF IATHFPHQVI LGVIGGMLVA EAFEHTPGIQ TASLGTYLKT NLFLFLFAVG FYLLLRVLNI DLLWSVPIAK KWCANPDWIH IDTTPFAGLV RNLGVLFGLG FAINSEMFLL SCRGGNNYTL SFRLLCALTS LTILQLYHFL QIPTHEEHLF YVLSFCKSAS IPLTVVAFIP YSVHMLMKQS GKKSQGSSGTETSQV APA
Sequence including C-terminal rho1D4 tag.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human G6PC2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
  • Tag Location: C-terminal rho1D4 tag.
Purification The protein is purified in three steps from baculovirus infected SF9 insect cells. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot. The best performing detergents - Fos-Choline 14 and DDM - are used for solubilization and G6PC2 is purified via the C-terminal rho1D4 tag. Eluate fractions are analyzed by Western blot. Protein containing fractions of the best purification are subjected to a second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Protein is endotoxin-free.
Grade Crystallography grade
Protein Name
Background May hydrolyze glucose-6-phosphate to glucose in the endoplasmic reticulum. May be responsible for glucose production through glycogenolysis and gluconeogenesis (By similarity). {ECO:0000250}.
Molecular Weight 41.8 kDa Including tag.
UniProt Q9NQR9
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
Comment

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
 image for Glucose-6-Phosphatase, Catalytic, 2 (G6PC2) (AA 1-355) protein (rho-1D4 tag) (ABIN3080375) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
Western Blotting (WB) image for Glucose-6-Phosphatase, Catalytic, 2 (G6PC2) (AA 1-355) protein (rho-1D4 tag) (ABIN3080375) Glucose-6-Phosphatase, Catalytic, 2 (G6PC2) (AA 1-355) protein (rho-1D4 tag) (Image 2)
Western Blotting (WB) image for Glucose-6-Phosphatase, Catalytic, 2 (G6PC2) (AA 1-355) protein (rho-1D4 tag) (ABIN3080375) Glucose-6-Phosphatase, Catalytic, 2 (G6PC2) (AA 1-355) protein (rho-1D4 tag) (Image 3)
Size-exclusion chromatography-High Pressure Liquid Chromatography (SEC-HPLC) image for Glucose-6-Phosphatase, Catalytic, 2 (G6PC2) (AA 1-355) protein (rho-1D4 tag) (ABIN3080375) Glucose-6-Phosphatase, Catalytic, 2 (G6PC2) (AA 1-355) protein (rho-1D4 tag) (Image 4)
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