Glutathione Synthetase Protein (GSS) (AA 2-474) (His tag)

Catalog No. ABIN3081179

Product Details

Target: Glutathione Synthetase (GSS)

Protein Type: Recombinant

Protein Characteristics: AA 2-474

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Glutathione Synthetase protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: ATNWGSLLQD KQQLEELARQ AVDRALAEGV LLRTSQEPTS SEVVSYAPFT LFPSLVPSAL LEQAYAVQMD FNLLVDAVSQ NAAFLEQTLS STIKQDDFTA RLFDIHKQVL KEGIAQTVFL GLNRSDYMFQ RSADGSPALK QIEINTISAS FGGLASRTPA VHRHVLSVLS KTKEAGKILS NNPSKGLALG IAKAWELYGS PNALVLLIAQ EKERNIFDQR AIENELLARN IHVIRRTFED ISEKGSLDQD RRLFVDGQEI AVVYFRDGYM PRQYSLQNWE ARLLLERSHA AKCPDIATQL AGTKKVQQEL SRPGMLEMLL PGQPEAVARL RATFAGLYSL DVGEEGDQAI AEALAAPSRF VLKPQREGGG NNLYGEEMVQ ALKQLKDSEE RASYILMEKI EPEPFENCLL RPGSPARVVQ CISELGIFGV YVRQEKTLVM NKHVGHLLRT KAIEHADGGV AAGVAVLDNP YPV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human GSS Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Glutathione Synthetase (GSS)

Alternative Name: GSS (GSS Products)

Synonyms: PRNP Protein, 6835 Protein, CG32497 Protein, CG33065 Protein, CG6835 Protein, Dmel\\CG6835 Protein, F21A20.90 Protein, F21A20_90 Protein, GLUTATHIONE SYNTHETASE Protein, GSHB Protein, glutathione synthetase 2 Protein, GSHS Protein, AI314904 Protein, GS-A/GS-B Protein, gssls-a Protein, glutathione synthetase Protein, Glutathione Synthetase Protein, glutathione synthetase 2 Protein, glutathione synthetase L homeolog Protein, GSS Protein, GSH2 Protein, Tola_1250 Protein, PC1_3711 Protein, Dd586_3633 Protein, Kvar_0701 Protein, Alvin_0197 Protein, Nhal_0215 Protein, BC1002_0254 Protein, Cseg_4017 Protein, Snov_3844 Protein, Aazo_1904 Protein, M301_2613 Protein, Bresu_0515 Protein, Isop_1460 Protein, Marme_4020 Protein, Thicy_0325 Protein, Sinme_3668 Protein, Mesop_0757 Protein, Gss Protein, gss.L Protein

Molecular Weight: 53.2 kDa Including tag.

UniProt: P48637

Pathways: Warburg Effect