Cytokeratin 18 Protein (AA 2-430) (His tag)

Catalog No. ABIN3082307

Product Details

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Protein Type: Recombinant

Protein Characteristics: AA 2-430

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Cytokeratin 18 protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: SFTTRSTFST NYRSLGSVQA PSYGARPVSS AASVYAGAGG SGSRISVSRS TSFRGGMGSG GLATGIAGGL AGMGGIQNEK ETMQSLNDRL ASYLDRVRSL ETENRRLESK IREHLEKKGP QVRDWSHYFK IIEDLRAQIF ANTVDNARIV LQIDNARLAA DDFRVKYETE LAMRQSVEND IHGLRKVIDD TNITRLQLET EIEALKEELL FMKKNHEEEV KGLQAQIASS GLTVEVDAPK SQDLAKIMAD IRAQYDELAR KNREELDKYW SQQIEESTTV VTTQSAEVGA AETTLTELRR TVQSLEIDLD SMRNLKASLE NSLREVEARY ALQMEQLNGI LLHLESELAQ TRAEGQRQAQ EYEALLNIKV KLEAEIATYR RLLEDGEDFN LGDALDSSNS MQTIQKTTTR RIVDGKVVSE TNDTKVLRH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human KRT18 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: KRT18 (KRT18 Products)

Synonyms: CK18 Protein, K18 Protein, Krt1-18 Protein, CYK18 Protein, DreK18 Protein, cb83 Protein, dapk1 Protein, sb:cb83 Protein, wu:fa13f02 Protein, wu:fb36b04 Protein, krt18 Protein, MGC64569 Protein, MGC75922 Protein, KRT18 Protein, CK-18-A Protein, K18-A Protein, NC-11 Protein, cyk18 Protein, k18 Protein, krt18-a Protein, krt18-b Protein, krt18a Protein, xkendob Protein, keratin 18 Protein, keratin 18, type I L homeolog Protein, keratin 18, type I Protein, keratin, type I cytoskeletal 18 Protein, K18, simple type I keratin Protein, Keratin, type I cytoskeletal 18 Protein, keratin 18, type I S homeolog Protein, Krt18 Protein, KRT18 Protein, krt18 Protein, krt18.L Protein, LOC700569 Protein, LOC100136778 Protein, k1c18 Protein, LOC100008885 Protein, krt18.S Protein

Background: Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. {ECO:0000250, ECO:0000269|PubMed:15529338, ECO:0000269|PubMed:16424149, ECO:0000269|PubMed:17213200, ECO:0000269|PubMed:7523419, ECO:0000269|PubMed:8522591, ECO:0000269|PubMed:9298992, ECO:0000269|PubMed:9524113}.

Molecular Weight: 48.9 kDa Including tag.

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis