MED27 Protein (AA 1-311) (Strep Tag)

Catalog No. ABIN3083534

Product Details

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Protein Type: Recombinant

Protein Characteristics: AA 1-311

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This MED27 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MADVINVSVN LEAFSQAISA IQALRSSVSR VFDCLKDGMR NKETLEGREK AFIAHFQDNL HSVNRDLNEL ERLSNLVGKP SENHPLHNSG LLSLDPVQDK TPLYSQLLQA YKWSNKLQYH AGLASGLLNQ QSLKRSANQM GVSAKRRPKA QPTTLVLPPQ YVDDVISRID RMFPEMSIHL SRPNGTSAML LVTLGKVLKV IVVMRSLFID RTIVKGYNEN VYTEDGKLDI WSKSNYQVFQ KVTDHATTAL LHYQLPQMPD VVVRSFMTWL RSYIKLFQAP CQRCGKFLQD GLPPTWRDFR TLEAFHDTCR Q
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Alternative Name: MED27 (MED27 Products)

Synonyms: CRAP34 Protein, CRSP34 Protein, CRSP8 Protein, TRAP37 Protein, CG1245 Protein, Dmel\\CG1245 Protein, Med27 Protein, Trap37 Protein, dCRSP34 Protein, dCrsp34 Protein, dTRAP37 Protein, med27 Protein, crsp8 Protein, zgc:66302 Protein, MED27 Protein, DDBDRAFT_0216764 Protein, DDBDRAFT_0266847 Protein, DDB_0216764 Protein, DDB_0266847 Protein, 1500015J03Rik Protein, 2310042P07Rik Protein, AA682045 Protein, Crsp8 Protein, D2Ertd434e Protein, RGD1564993 Protein, DKFZp459I1912 Protein, CRSP complex subunit 8-B Protein, Xcrsp34 Protein, crap34 Protein, crsp34 Protein, crsp8-B Protein, med27-a Protein, med27-b Protein, trap37 Protein, mediator complex subunit 27 Protein, Mediator complex subunit 27 Protein, cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa Protein, hypothetical protein Protein, mediator complex subunit 27 S homeolog Protein, MED27 Protein, med27 Protein, crsp8 Protein, Med27 Protein, med27.S Protein

Background: Mediator of RNA polymerase II transcription subunit 27 (Cofactor required for Sp1 transcriptional activation subunit 8) (CRSP complex subunit 8) (Mediator complex subunit 27) (P37 TRAP/SMCC/PC2 subunit) (Transcriptional coactivator CRSP34),FUNCTION: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. {ECO:0000269|PubMed:10882111, ECO:0000269|PubMed:9989412}.

Molecular Weight: 35.4 kDa

UniProt: Q6P2C8

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha