NUP62 Protein (AA 2-522) (His tag)

Catalog No. ABIN3084767

Product Details

Target: NUP62 (Nucleoporin 62kDa (NUP62))

Protein Type: Recombinant

Protein Characteristics: AA 2-522

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This NUP62 protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: SGFNFGGTGA PTGGFTFGTA KTATTTPATG FSFSTSGTGG FNFGAPFQPA TSTPSTGLFS LATQTPATQT TGFTFGTATL ASGGTGFSLG IGASKLNLSN TAATPAMANP SGFGLGSSNL TNAISSTVTS SQGTAPTGFV FGPSTTSVAP ATTSGGFSFT GGSTAQPSGF NIGSAGNSAQ PTAPATLPFT PATPAATTAG ATQPAAPTPT ATITSTGPSL FASIATAPTS SATTGLSLCT PVTTAGAPTA GTQGFSLKAP GAASGTSTTT STAATATATT TSSSSTTGFA LNLKPLAPAG IPSNTAAAVT APPGPGAAAG AAASSAMTYA QLESLINKWS LELEDQERHF LQQATQVNAW DRTLIENGEK ITSLHREVEK VKLDQKRLDQ ELDFILSQQK ELEDLLSPLE ELVKEQSGTI YLQHADEERE KTYKLAENID AQLKRMAQDL KDIIEHLNTS GAPADTSDPL QQICKILNAH MDSLQWIDQN SALLQRKVEE VTKVCEGRRK EQERSFRITF D
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human NUP62 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: NUP62 (Nucleoporin 62kDa (NUP62))

Alternative Name: NUP62 (NUP62 Products)

Synonyms: IBSN Protein, SNDI Protein, p62 Protein, NUP62 Protein, AA589433 Protein, AI426861 Protein, AI790512 Protein, AU045898 Protein, D7Ertd649e Protein, Nupc1 Protein, CG6251 Protein, Dmel\\CG6251 Protein, DDBDRAFT_0167902 Protein, DDBDRAFT_0237712 Protein, DDB_0167902 Protein, DDB_0237712 Protein, nup62 Protein, Np62 Protein, nucleoporin 62 Protein, hypothetical protein Protein, nucleoporin 62kDa Protein, Nucleoporin 62kD Protein, predicted protein Protein, nuclear pore protein Protein, Nup62 nuclear pore complex glycoprotein p62-related protein (IC) Protein, Nuclear pore glycoprotein p62 Protein, NUP62 Protein, PGTG_03077 Protein, Nup62 Protein, nup62 Protein

Background: Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex.

Molecular Weight: 54.1 kDa Including tag.

UniProt: P37198

Pathways: EGFR Signaling Pathway, SARS-CoV-2 Protein Interactome