Slc6a14 Protein (AA 1-642) (Strep Tag)

Catalog No. ABIN3087667

Product Details

Target: Slc6a14 (Solute Carrier Family 6 (Amino Acid Transporter), Member 14 (Slc6a14))

Protein Type: Recombinant

Protein Characteristics: AA 1-642

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This Slc6a14 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MDKLKCPSFF KCREKEKVSA SSENFHVGEN DENQDRGNWS KKSDYLLSMI GYAVGLGNVW RFPYLTYSNG GGAFLIPYAI MLALAGLPLF FLECSLGQFA SLGPVSVWRI LPLFQGVGIT MVLISIFVTI YYNVIIAYSL YYMFASFQSE LPWKNCSSWS DKNCSRSPIV THCNVSTVNK GIQEIIQMNK SWVDINNFTC INGSEIYQPG QLPSEQYWNK VALQRSSGMN ETGVIVWYLA LCLLLAWLIV GAALFKGIKS SGKVVYFTAL FPYVVLLILL VRGATLEGAS KGISYYIGAQ SNFTKLKEAE VWKDAATQIF YSLSVAWGGL VALSSYNKFK NNCFSDAIVV CLTNCLTSVF AGFAIFSILG HMAHISGKEV SQVVKSGFDL AFIAYPEALA QLPGGPFWSI LFFFMLLTLG LDSQFASIET ITTTIQDLFP KVMKKMRVPI TLGCCLVLFL LGLVCVTQAG IYWVHLIDHF CAGWGILIAA ILELVGIIWI YGGNRFIEDT EMMIGAKRWI FWLWWRACWF VITPILLIAI FIWSLVQFHR PNYGAIPYPD WGVALGWCMI VFCIIWIPIM AIIKIIQAKG NIFQRLISCC RPASNWGPYL EQHRGERYKD MVDPKKEADH EIPTVSGSRK PE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Slc6a14 (Solute Carrier Family 6 (Amino Acid Transporter), Member 14 (Slc6a14))

Alternative Name: SLC6A14 (Slc6a14 Products)

Synonyms: BMIQ11 Protein, 1110007A17Rik Protein, 9030613J17Rik Protein, ATB0plus Protein, CATB0plus Protein, solute carrier family 6 member 14 Protein, solute carrier family 6 (neurotransmitter transporter), member 14 Protein, SLC6A14 Protein, Slc6a14 Protein

Background: Sodium- and chloride-dependent neutral and basic amino acid transporter B(0+) (Amino acid transporter ATB0+) (Solute carrier family 6 member 14),FUNCTION: Amino acid transporter that plays an important role in the absorption of amino acids in the intestinal tract. Mediates the uptake of a broad range of neutral and cationic amino acids (with the exception of proline) in a Na(+)/Cl(-)-dependent manner (PubMed:10446133). Transports non-alpha-amino acids such as beta-alanine with low affinity, and has a higher affinity for dipolar and cationic amino acids such as leucine and lysine (PubMed:18599538). Can also transport carnitine, butirylcarnitine and propionylcarnitine coupled to the transmembrane gradients of Na(+) and Cl(-) (PubMed:17855766). {ECO:0000250|UniProtKB:Q9JMA9, ECO:0000269|PubMed:10446133, ECO:0000269|PubMed:17855766, ECO:0000269|PubMed:18599538}.

Molecular Weight: 72.2 kDa

UniProt: Q9UN76