SLC25A46 Protein (AA 1-418) (Strep Tag)

Catalog No. ABIN3087751

Product Details

Target: SLC25A46 (Solute Carrier Family 25, Member 46 (SLC25A46))

Protein Type: Recombinant

Protein Characteristics: AA 1-418

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SLC25A46 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MHPRRPDGFD GLGYRGGARD EQGFGGAFPA RSFSTGSDLG HWVTTPPDIP GSRNLHWGEK SPPYGVPTTS TPYEGPTEEP FSSGGGGSVQ GQSSEQLNRF AGFGIGLASL FTENVLAHPC IVLRRQCQVN YHAQHYHLTP FTVINIMYSF NKTQGPRALW KGMGSTFIVQ GVTLGAEGII SEFTPLPREV LHKWSPKQIG EHLLLKSLTY VVAMPFYSAS LIETVQSEII RDNTGILECV KEGIGRVIGM GVPHSKRLLP LLSLIFPTVL HGVLHYIISS VIQKFVLLIL KRKTYNSHLA ESTSPVQSML DAYFPELIAN FAASLCSDVI LYPLETVLHR LHIQGTRTII DNTDLGYEVL PINTQYEGMR DCINTIRQEE GVFGFYKGFG AVIIQYTLHA AVLQITKIIY STLLQNNI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SLC25A46 (Solute Carrier Family 25, Member 46 (SLC25A46))

Alternative Name: SLC25A46 (SLC25A46 Products)

Synonyms: SLC25A46 Protein, MGC152354 Protein, zgc:92767 Protein, slc25a46 Protein, 1200007B05Rik Protein, AI325987 Protein, RGD1305072 Protein, solute carrier family 25 member 46 Protein, solute carrier family 25, member 46 Protein, solute carrier family 25 member 46 L homeolog Protein, SLC25A46 Protein, slc25a46 Protein, slc25a46.L Protein, Slc25a46 Protein

Background: Mitochondrial outer membrane protein SLC25A46 (Solute carrier family 25 member 46),FUNCTION: Transmembrane protein of the mitochondrial outer membrane that controls mitochondrial organization (PubMed:26168012, PubMed:27543974, PubMed:27390132). May regulate the assembly of the MICOS (mitochondrial contact site and cristae organizing system) complex which is essential to the biogenesis and dynamics of mitochondrial cristae, the inwards folds of the inner mitochondrial membrane (PubMed:27390132). Through its interaction with the EMC (endoplasmic reticulum membrane protein complex), could regulate mitochondrial lipid homeostasis and thereby mitochondrial fission (PubMed:27390132). {ECO:0000269|PubMed:26168012, ECO:0000269|PubMed:27390132, ECO:0000269|PubMed:27543974}.

Molecular Weight: 46.2 kDa

UniProt: Q96AG3