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AHCY Protein (AA 2-432) (His tag)

Crystallography grade AHCY Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Catalog No. ABIN3087827
  • Target See all AHCY Proteins
    AHCY (Adenosylhomocysteinase (AHCY))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-432
    Origin
    • 7
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    Human
    Source
    • 61
    • 6
    • 1
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This AHCY protein is labelled with His tag.
    Application
    ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
    Sequence
    SDKLPYKVAD IGLAAWGRKA LDIAENEMPG LMRMRERYSA SKPLKGARIA GCLHMTVETA VLIETLVTLG AEVQWSSCNI FSTQDHAAAA IAKAGIPVYA WKGETDEEYL WCIEQTLYFK DGPLNMILDD GGDLTNLIHT KYPQLLPGIR GISEETTTGV HNLYKMMANG ILKVPAINVN DSVTKSKFDN LYGCRESLID GIKRATDVMI AGKVAVVAGY GDVGKGCAQA LRGFGARVII TEIDPINALQ AAMEGYEVTT MDEACQEGNI FVTTTGCIDI ILGRHFEQMK DDAIVCNIGH FDVEIDVKWL NENAVEKVNI KPQVDRYRLK NGRRIILLAE GRLVNLGCAM GHPSFVMSNS FTNQVMAQIE LWTHPDKYPV GVHFLPKKLD EAVAEAHLGK LNVKLTKLTE KQAQYLGMSC DGPFKPDHYR Y
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human AHCY Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product AHCY Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    AHCY (Adenosylhomocysteinase (AHCY))
    Alternative Name
    AHCY (AHCY Products)
    Synonyms
    sahh Protein, PSPTO5068 Protein, Ahcy Protein, SAHH Protein, adoHcyase Protein, ahcy Protein, AA987153 Protein, AL024110 Protein, CuBP Protein, D150 Protein, cb1079 Protein, hm:zeh1173 Protein, hm:zeh1364 Protein, wu:fj67b02 Protein, adenosylhomocysteinase Protein, Adenosylhomocysteinase Protein, S-adenosylhomocysteine hydrolase Protein, adenosylhomocysteinase S homeolog Protein, AHCY Protein, ahcy Protein, ahcY Protein, MMAH_RS02605 Protein, Srot_2217 Protein, Ndas_3755 Protein, Deba_0936 Protein, Palpr_2392 Protein, Calni_0569 Protein, LOC100282150 Protein, Intca_2510 Protein, Marky_0392 Protein, Desac_1394 Protein, Tc00.1047053511229.50 Protein, Tc00.1047053511589.200 Protein, Tb11.01.1350 Protein, LMJF_36_3910 Protein, MCYG_06244 Protein, Ahcy Protein, ahcy.S Protein
    Background
    Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions, therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. {ECO:0000269|PubMed:12590576}.
    Molecular Weight
    48.5 kDa Including tag.
    UniProt
    P23526
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