14-3-3 zeta Protein (YWHAZ) (AA 1-245) (Strep Tag)

Catalog No. ABIN3087997

Product Details

Target: 14-3-3 zeta (YWHAZ)

Protein Type: Recombinant

Protein Characteristics: AA 1-245

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This 14-3-3 zeta protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MDKNELVQKA KLAEQAERYD DMAACMKSVT EQGAELSNEE RNLLSVAYKN VVGARRSSWR VVSSIEQKTE GAEKKQQMAR EYREKIETEL RDICNDVLSL LEKFLIPNAS QAESKVFYLK MKGDYYRYLA EVAAGDDKKG IVDQSQQAYQ EAFEISKKEM QPTHPIRLGL ALNFSVFYYE ILNSPEKACS LAKTAFDEAI AELDTLSEES YKDSTLIMQL LRDNLTLWTS DTQGDEAEAG EGGEN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: 14-3-3 zeta (YWHAZ)

Alternative Name: YWHAZ (YWHAZ Products)

Synonyms: 14-3-3-zeta Protein, KCIP-1 Protein, YWHAD Protein, 14-3-3zeta Protein, Ywhaz Protein, ACYPI003154 Protein, 14-3-3z Protein, kcip-1 Protein, ywhaq Protein, 1433z Protein, ywhaz Protein, ywhazb Protein, 1110013I11Rik Protein, AI596267 Protein, AL022924 Protein, AU020854 Protein, ywhaza Protein, fb14h09 Protein, wu:fb05g08 Protein, wu:fb14h09 Protein, ywhai Protein, zgc:55807 Protein, 14-3-3 Protein, 14-3-3 zeta Protein, 14-3-3ZETA Protein, 14-3-3leo Protein, 2G1 Protein, 4-3-3 zeta Protein, 5.11 Protein, 549 Protein, BEST:GH05075 Protein, CG17870 Protein, D14-3-3 Protein, D14-3-3zeta Protein, Dmel\\CG17870 Protein, K Protein, LEO Protein, Leo Protein, PAR-5 Protein, PAR5 Protein, Par-5 Protein, d14-3-3zeta Protein, l(2)07103 Protein, l(2)46CFe Protein, l(2)46Ee Protein, leo Protein, par-5 Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta Protein, 14-3-3 protein zeta Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta L homeolog Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta S homeolog Protein, 14-3-3 protein zeta/delta pseudogene Protein, CG17870 gene product from transcript CG17870-RE Protein, YWHAZ Protein, 14-3-3zeta Protein, ywhaz Protein, 1433z Protein, ywhaz.L Protein, Ywhaz Protein, ywhaz.S Protein, LOC100855903 Protein

Background: 14-3-3 protein zeta/delta (Protein kinase C inhibitor protein 1) (KCIP-1),FUNCTION: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:14578935, PubMed:15071501, PubMed:15644438, PubMed:16376338, PubMed:16959763, PubMed:31024343, PubMed:9360956). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:35662396). Binding generally results in the modulation of the activity of the binding partner (PubMed:35662396). Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB (PubMed:35662396). Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation (PubMed:16959763). In neurons, regulates spine maturation through the modulation of ARHGEF7 activity (By similarity). {ECO:0000250|UniProtKB:O55043, ECO:0000269|PubMed:14578935, ECO:0000269|PubMed:15071501, ECO:0000269|PubMed:15644438, ECO:0000269|PubMed:16376338, ECO:0000269|PubMed:16959763, ECO:0000269|PubMed:31024343, ECO:0000269|PubMed:35662396, ECO:0000269|PubMed:9360956}.

Molecular Weight: 27.7 kDa

UniProt: P63104

Pathways: Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location