YWHAE Protein (AA 1-255) (Strep Tag)

Catalog No. ABIN3088245

Product Details

Target: YWHAE (14-3-3 epsilon (YWHAE))

Protein Type: Recombinant

Protein Characteristics: AA 1-255

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This YWHAE protein is labelled with Strep Tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Sequence: x

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: YWHAE (14-3-3 epsilon (YWHAE))

Alternative Name: YWHAE (YWHAE Products)

Synonyms: 14-3-3 Protein, 14-3-3 epsilon Protein, 14-3-3-e Protein, 14-3-3-epsilon Protein, 14-3-30epsilon Protein, 14-3-3EPSILON Protein, 14-3-3e Protein, 14-3-3omicron Protein, 1433epsilon Protein, 143E_DROME Protein, CG31196 Protein, CT24092 Protein, D14-3-3e Protein, D14-3-3epsilon Protein, Dmel\\CG31196 Protein, EK3-5 Protein, PAR-5 Protein, PAR5 Protein, Par-5 Protein, SR3-9 Protein, Su(Raf)3B Protein, anon-WO0172774.141 Protein, anon-WO02059370.52 Protein, d14-3-3epsilon Protein, eps Protein, l(3)j2B10 Protein, par-5 Protein, 14-3-3epsilon Protein, kcip-1 Protein, mdcr Protein, mds Protein, LOC581376 Protein, Ywhae Protein, ACYPI002517 Protein, YWHAE Protein, 14-3-3E Protein, KCIP-1 Protein, MDCR Protein, MDS Protein, AU019196 Protein, fe38c12 Protein, wu:fb48f06 Protein, wu:fe38c12 Protein, wu:fi22e10 Protein, ywhae Protein, zgc:55377 Protein, zgc:77062 Protein, CG31196 gene product from transcript CG31196-RD Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon Protein, 14-3-3 epsilon Protein, 14-3-3 epsilon protein Protein, 14-3-3 protein epsilon Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon S homeolog Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide 1 Protein, 14-3-3epsilon Protein, ywhae Protein, LOC581376 Protein, Ywhae Protein, HCAG_04173 Protein, PTRG_02836 Protein, PTRG_04459 Protein, UREG_07864 Protein, YWHAE Protein, ywhae.S Protein, ywhae1 Protein

Background: 14-3-3 protein epsilon (14-3-3E),FUNCTION: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:35343654). Binding generally results in the modulation of the activity of the binding partner (By similarity). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326). Plays a positive role in the antiviral signaling pathway upstream of TBK1 via interaction with RIGI (PubMed:37555661). Mechanistically, directs RIGI redistribution from the cytosol to mitochondrial associated membranes where it mediates MAVS-dependent innate immune signaling during viral infection (PubMed:22607805). Plays a role in proliferation inhibition and cell cycle arrest by exporting HNRNPC from the nucleus to the cytoplasm to be degraded by ubiquitination (PubMed:37599448). {ECO:0000250|UniProtKB:P62261, ECO:0000269|PubMed:12917326, ECO:0000269|PubMed:22607805, ECO:0000269|PubMed:35343654, ECO:0000269|PubMed:37555661, ECO:0000269|PubMed:37599448}.

Molecular Weight: 29.2 kDa

UniProt: P62258

Pathways: Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase