Cholinergic Receptor, Nicotinic, epsilon (Muscle) (CHRNE) (AA 21-239) protein (His tag)

Details for Product No. ABIN3088543
Protein Name
  • ACHRE
  • CMS1D
  • CMS1E
  • CMS2A
  • FCCMS
  • SCCMS
  • AChrepsilon
  • Acre
  • nAChRE
  • im:7154697
  • zgc:101010
  • CHRNE
  • cholinergic receptor nicotinic epsilon subunit
  • cholinergic receptor, nicotinic, epsilon polypeptide
  • cholinergic receptor, nicotinic epsilon L homeolog
  • cholinergic receptor, nicotinic, epsilon
  • CHRNE
  • Chrne
  • chrne.L
  • chrne
Protein Characteristics
AA 21-239
2
2
2
1
Origin
Human
5
3
Source
Escherichia coli (E. coli)
4
3
1
Protein Type
Recombinant
Purification tag / Conjugate
This CHRNE protein is labelled with His tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Options
Sequence MHHHHHH KNEELRLYHH LFNNYDPGSR PVREPEDTVT ISLKVTLTNL ISLNEKEETL TTSVWIGIDW QDYRLNYSKD DFGGIETLRV PSELVWLPEI VLENNIDGQF GVAYDANVLV YEGGSVTWLP PAIYRSVCAV EVTYFPFDWQ NCSLIFRSQT YNAEEVEFTF AVDNDGKTIN KIDIDTEAYT ENGEWAIDFC PGVIRRHHGG ATDGPGETDV IYSLIIRRK
Sequence includes N-terminal His-tag
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human PLP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification The protein is purified from the cleared cell lysate using His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Endotoxin has not been removed. Please contact us if you require endotoxin removal.
Grade Crystallography grade
Protein Name
Background After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Molecular Weight 26.0 kDa Including tag
UniProt Q04844
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
Comment

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer In solution (30 mM Hepes, pH 8.0, 100 mM NaCl, Laurylsarcosine)
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
Image no. 1 for Cholinergic Receptor, Nicotinic, epsilon (Muscle) (CHRNE) (AA 21-239) protein (His tag) (ABIN3088543) Cholinergic Receptor, Nicotinic, epsilon (Muscle) (CHRNE) (AA 21-239) protein (His tag) (Image 1)
Image no. 2 for Cholinergic Receptor, Nicotinic, epsilon (Muscle) (CHRNE) (AA 21-239) protein (His tag) (ABIN3088543) Cholinergic Receptor, Nicotinic, epsilon (Muscle) (CHRNE) (AA 21-239) protein (His tag) (Image 2)
Product cited in: Chernyavsky, Amber, Agnoletti, Wang, Grando: "Synergy among non-desmoglein antibodies contributes to the immunopathology of desmoglein antibody-negative pemphigus vulgaris." in: The Journal of biological chemistry, Vol. 294, Issue 12, pp. 4520-4528, 2019 (PubMed).

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