SSX2IP Protein (AA 1-614) (Strep Tag)

Catalog No. ABIN3088583

Product Details

Target: SSX2IP (Synovial Sarcoma, X Breakpoint 2 Interacting Protein (SSX2IP))

Protein Type: Recombinant

Protein Characteristics: AA 1-614

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SSX2IP protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MGDWMTVTDP GLSSESKTIS QYTSETKMSP SSLYSQQVLC SSIPLSKNVH SFFSAFCTED NIEQSISYLD QELTTFGFPS LYEESKGKET KRELNIVAVL NCMNELLVLQ RKNLLAQENV ETQNLKLGSD MDHLQSCYSK LKEQLETSRR EMIGLQERDR QLQCKNRNLH QLLKNEKDEV QKLQNIIASR ATQYNHDMKR KEREYNKLKE RLHQLVMNKK DKKIAMDILN YVGRADGKRG SWRTGKTEAR NEDEMYKILL NDYEYRQKQI LMENAELKKV LQQMKKEMIS LLSPQKKKPR ERVDDSTGTV ISDVEEDAGE LSRESMWDLS CETVREQLTN SIRKQWRILK SHVEKLDNQV SKVHLEGFND EDVISRQDHE QETEKLELEI QQCKEMIKTQ QQLLQQQLAT AYDDDTTSLL RDCYLLEEKE RLKEEWSLFK EQKKNFERER RSFTEAAIRL GLERKAFEEE RASWLKQQFL NMTTFDHQNS ENVKLFSAFS GSSDWDNLIV HSRQPQKKPH SVSNGSPVCM SKLTKSLPAS PSTSDFCQTR SCISEHSSIN VLNITAEEIK PNQVGGECTN QKWSVASRPG SQEGCYSGCS LSYTNSHVEK DDLP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SSX2IP (Synovial Sarcoma, X Breakpoint 2 Interacting Protein (SSX2IP))

Alternative Name: SSX2IP (SSX2IP Products)

Synonyms: zgc:73314 Protein, MGC83757 Protein, ADIP Protein, LCG Protein, AU014939 Protein, AU042321 Protein, Adip Protein, synovial sarcoma, X breakpoint 2 interacting protein a Protein, synovial sarcoma, X breakpoint 2 interacting protein S homeolog Protein, SSX family member 2 interacting protein Protein, synovial sarcoma, X 2 interacting protein Protein, ssx2ipa Protein, ssx2ip.S Protein, SSX2IP Protein, Ssx2ip Protein

Background: Afadin- and alpha-actinin-binding protein (ADIP) (Afadin DIL domain-interacting protein) (SSX2-interacting protein),FUNCTION: Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin (By similarity). Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling (By similarity). Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73, the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome (PubMed:23816619, PubMed:26545777). Involved in ciliogenesis (PubMed:24356449). It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia (PubMed:24356449). {ECO:0000250|UniProtKB:Q8VC66, ECO:0000269|PubMed:23816619, ECO:0000269|PubMed:24356449, ECO:0000305|PubMed:26545777}.

Molecular Weight: 71.2 kDa

UniProt: Q9Y2D8