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ANKRD5 Protein (AA 1-776) (His tag)

Crystallography grade ANKRD5 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3088743
  • Target See all ANKRD5 products
    ANKRD5 (Ankyrin Repeat Domain 5 (ANKRD5))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-776
    Origin
    • 1
    • 1
    Human
    Source
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This ANKRD5 protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MALADKRLEN LQIYKVLQCV RNKDKKQIEK LTKLGYPELI NYTEPINGLS ALHLASVSND IDMVSFLLDL GAHPDVQDRM GCTPTMRAAE LGHELSMEIL AKAKADMTIV DNEGKGVLFY CILPTKRHYR CALIALEHGA DVNNSTYEGK PIFLRACEDA HDVKDVCLTF LEKGANPNAI NSSTGRTALM EASREGVVEI VRGILERGGE VNAFDNDRHH AAHFAAKGGF FDILKLLFAY NGDVGLISIN GNTPLHYAAM GGFADCCKYI AQRGCDLKWK NLDHKTPRAV AKEGGFKAAS KEIRRAERIA NKLARPGAKN PNPLWALRLH DWSVEREAFL REAFAVLDRG DGSISKNDFV MVLEERQDYA SSEQLAAIAH LHEKTRGGGV NINEFFKGTR YLNKSFVLGS YGPKKKEKGM GKKGKKGKFV LPLPICVIPE YAFPRRQDGG PPYYMIETYK NVTDSSRFNR DHPPEHPIQD DSVWYIDDSE KVFSNINIIT KAGDLASLKK AFESGIPVDM KDNYYKTPLM TACASGNIDV VKFLLEKGAN VNATDNFLWT PLHFACHAGQ QDIVELLVES GALIDAASIN NSTPLNRAIE SCRLDTVKYL LDIGAKFQLE NRKGHSAMDV AKAYADYRII DLIKEKLDNL PKPAENQKLK GKTPPILKTE GPEIKKEEEL LSSIYGVPTT SEGKKVQKGN VVHLNSLITS GYTKKVDITF IPRRIWSPEA TTAELIRKRE LRRERFTHEV DFDDFMMPFQ KNITEKARAL EAALKT
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ANKEF1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    ANKRD5 (Ankyrin Repeat Domain 5 (ANKRD5))
    Alternative Name
    ANKEF1 (ANKRD5 Products)
    Synonyms
    anr5 Protein, MGC146428 Protein, DKFZp469P046 Protein, ANKRD5 Protein, dJ839B4.6 Protein, AV276460 Protein, Ankrd5 Protein, ankyrin repeat and EF-hand domain containing 1 L homeolog Protein, ankyrin repeat and EF-hand domain containing 1 Protein, ankef1.L Protein, ankef1 Protein, ANKEF1 Protein, Ankef1 Protein
    Molecular Weight
    87.6 kDa Including tag.
    UniProt
    Q9NU02
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