Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

ANAPC11 Protein (AA 1-84) (His tag)

Crystallography grade ANAPC11 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3088831
  • Target See all ANAPC11 Proteins
    ANAPC11 (Anaphase Promoting Complex Subunit 11 (ANAPC11))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-84
    Origin
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 4
    • 1
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This ANAPC11 protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MKVKIKCWNG VATWLWVAND ENCGICRMAF NGCCPDCKVP GDDCPLVWGQ CSHCFHMHCI LKWLHAQQVQ QHCPMCRQEW KFKE
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ANAPC11 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product ANAPC11 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    ANAPC11 (Anaphase Promoting Complex Subunit 11 (ANAPC11))
    Alternative Name
    ANAPC11 (ANAPC11 Products)
    Synonyms
    APC11 Protein, Apc11p Protein, anapc11 Protein, RGD1561880 Protein, F10A16.17 Protein, F10A16_17 Protein, anaphase-promoting complex/cyclosome 11 Protein, DDBDRAFT_0187183 Protein, DDBDRAFT_0231277 Protein, DDB_0187183 Protein, DDB_0231277 Protein, 1110011I19Rik Protein, R75218 Protein, zgc:153671 Protein, si:dkey-237i9.4 Protein, anaphase promoting complex subunit 11 Protein, anaphase promoting complex subunit 11 L homeolog Protein, anaphase-promoting complex/cyclosome 11 Protein, anaphase-promoting complex subunit Apc11 Protein, ubiquitin-protein ligase Anaphase Promoting Complex Protein, hypothetical protein Protein, predicted protein Protein, anaphase-promoting complex subunit 11 Protein, APC11 anaphase promoting complex subunit 11 homolog (yeast) Protein, ANAPC11 Protein, anapc11.L Protein, anapc11 Protein, Anapc11 Protein, APC11 Protein, apc11 Protein, CAALFM_CR10610CA Protein, LOC9320604 Protein, LOC692923 Protein
    Background
    Together with the cullin protein ANAPC2, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. May recruit the E2 ubiquitin-conjugating enzymes to the complex. {ECO:0000269|PubMed:11739784, ECO:0000269|PubMed:18485873}.
    Molecular Weight
    10.8 kDa Including tag.
    UniProt
    Q9NYG5
You are here:
Support